Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.).
Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.).
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DOI：
10.3389/fpls.2022.866276
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
传递比畸变（TRD）是指在杂合子后代中观察到的等位基因或基因型频率偏离预期的孟德尔分离比。TRD会严重阻碍水稻种间和种内的基因流动。在这里，我们报告了精细的定位和表征的两个位点（TRD 4.1和TRD 4.2）TRD使用大型F2分离群体，这是来自水稻染色体片段置换线，每个包含一个特定的基因组片段从粳稻品种日本晴（NIP）到籼稻品种珍汕（97）。这两个基因座在后代中表现出优先传递1097等位基因。使用在TRD 4.1处具有三个不同等位基因的近等基因系的互交实验表明，该基因引起雄性配子选择。此外，当杂合子携带纯合子TRD4.1时，TRD4.2的传递偏倚在杂合子中减少。这表明这两个基因座之间存在上位相互作用。TRD 4.2定位在一个35-kb的区域内，该区域包含一个在水稻生殖器官中特异表达的候选基因。这些发现拓宽了对TRD遗传机制的理解，并为克服水稻亚种间的基因流动障碍提供了一条途径，从而有助于通过渐渗育种进行水稻改良。
Transmission ratio distortion (TRD) denotes the observed allelic or genotypic frequency deviation from the expected Mendelian segregation ratios in the offspring of a heterozygote. TRD can severely hamper gene flow between and within rice species. Here, we report the fine mapping and characterization of two loci (TRD4.1 and TRD4.2) for TRD using large F2 segregating populations, which are derived from rice chromosome segment substitution lines, each containing a particular genomic segment introduced from the japonica cultivar Nipponbare (NIP) into the indica cultivar Zhenshan (ZS97). The two loci exhibited a preferential transmission of ZS97 alleles in the derived progeny. Reciprocal crossing experiments using near-isogenic lines harboring three different alleles at TRD4.1 suggest that the gene causes male gametic selection. Moreover, the transmission bias of TRD4.2 was diminished in heterozygotes when they carried homozygous TRD4.1ZS97. This indicates an epistatic interaction between these two loci. TRD4.2 was mapped into a 35-kb region encompassing one candidate gene that is specifically expressed in the reproductive organs in rice. These findings broaden the understanding of the genetic mechanisms of TRD and offer an approach to overcome the barrier of gene flow between the subspecies in rice, thus facilitating rice improvement by introgression breeding.
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DOI：
10.1186/s12284-014-0003-8
发表时间：
2014-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Reflinur;Kim B;Jang SM;Chu SH;Bordiya Y;Akter MB;Lee J;Chin JH;Koh HJ
通讯作者：
Koh HJ
影响因子：
5.4
作者：
Chandnani, Rahul;Wang, Baohua;Paterson, Andrew H.
通讯作者：
Paterson, Andrew H.
多连接双亲染色体片段取代系群体的代谢组分析
影响因子：
7.4
作者：
Chen, Jie;Wang, Jilin;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
3.1
作者：
Kumar, Sunil;Gill, Bikram S.;Faris, Justin D.
通讯作者：
Faris, Justin D.
三个代表性的亚种间和亚种内杂交揭示了水稻生殖隔离的遗传结构
影响因子：
7.2
作者：
Li, Guangwei;Li, Xiaoting;Ouyang, Yidan
通讯作者：
Ouyang, Yidan