An extensive circuitry for cell wall regulation in Candida albicans.
An extensive circuitry for cell wall regulation in Candida albicans.
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DOI：
10.1371/journal.ppat.1000752
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发表时间：
2010-02-05
期刊：
影响因子：
6.7
通讯作者：
Mitchell AP
中科院分区：
文献类型：
--
作者：
Blankenship JR;Fanning S;Hamaker JJ;Mitchell AP
蛋白激酶在信号传导和响应外部环境变化中起关键作用。白色念珠菌快速感知和响应环境变化的能力是其在人类宿主中生存的关键。我们的指导假设是，创建和筛选一组蛋白激酶突变株将揭示介导C.白色念珠菌建立了蛋白激酶突变株库，并筛选了对各种胁迫的敏感性。对于大多数测试的压力，压力反应在很大程度上是保守的C。白色念珠菌、酿酒酵母和粟酒裂殖酵母。然而，我们确定了8个蛋白激酶的作用，在细胞壁调节（CWR）的模式真菌酿酒酵母和粟酒裂殖酵母的功能，他们的直系同源物没有预期。这些蛋白激酶的保守作用的分析表明，细胞极性的建立是至关重要的CWR。此外，我们发现，septins，关键的萌芽，都是重要的生存和被错误定位的细胞壁应力。我们的研究表明蛋白激酶信号在C.白色念珠菌细胞壁完整。我们的研究表明，在某些情况下，这种扩张对细胞壁生物发生中的某些途径具有更大的重要性。在其他情况下，似乎信号通路已经重新连接细胞壁完整性反应。 白色念珠菌是人类的主要致病真菌。与大多数微生物一样，C.白色念珠菌被细胞壁包围和保护。这种细胞壁有两个目的：作为一个刚性的“外骨骼”，以防止细胞破裂，并提供一个表面，细胞可以与外部环境相互作用，同时保护细胞本身免受这种环境的影响。在模式真菌酿酒酵母中，这种结构的维持已经得到了很好的研究，但是以前的证据表明，C。白色念珠菌可能对这一过程有额外的输入。通过创建和测试突变株对细胞壁应激敏感性的文库，我们能够鉴定出许多保守基因，这些基因在这一过程中的作用不是它们的S。cerevisiae对应物。尽管这些基因中的一些以前与细胞壁完整性有关，但它们似乎对C.白色念珠菌。对于其他基因，它们在细胞壁完整性中的作用可能代表了一种新的联系。我们的研究结果提供了深入了解这种病原体细胞壁完整性的新方面，并为其更详细的分析奠定了基础。
Protein kinases play key roles in signaling and response to changes in the external environment. The ability of Candida albicans to quickly sense and respond to changes in its environment is key to its survival in the human host. Our guiding hypothesis was that creating and screening a set of protein kinase mutant strains would reveal signaling pathways that mediate stress response in C. albicans. A library of protein kinase mutant strains was created and screened for sensitivity to a variety of stresses. For the majority of stresses tested, stress response was largely conserved between C. albicans, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. However, we identified eight protein kinases whose roles in cell wall regulation (CWR) were not expected from functions of their orthologs in the model fungi Saccharomyces cerevisiae and Schizosaccharomyces pombe. Analysis of the conserved roles of these protein kinases indicates that establishment of cell polarity is critical for CWR. In addition, we found that septins, crucial to budding, are both important for surviving and are mislocalized by cell wall stress. Our study shows an expanded role for protein kinase signaling in C. albicans cell wall integrity. Our studies suggest that in some cases, this expansion represents a greater importance for certain pathways in cell wall biogenesis. In other cases, it appears that signaling pathways have been rewired for a cell wall integrity response. Candida albicans is the major fungal commensal and pathogen of humans. Like most microorganisms, C. albicans is surrounded and protected by a cell wall. This cell wall has two purposes: to act as a rigid “exoskeleton” to prevent cells from bursting, and to provide a surface where a cell can interact with the outside environment while protecting the cell itself from this environment. Maintenance of this structure has been well studied in the model fungus, Saccharomyces cerevisiae, but previous evidence suggested that C. albicans might have additional inputs to this process. By creating and testing a library of mutant strains for sensitivity to cell wall stress, we were able to identify a number of conserved genes with roles in this process not shared by their S. cerevisiae counterparts. Although some of these genes had previously been linked to cell wall integrity, it appears that they have increased impact on this process in C. albicans. For other genes, their role in cell wall integrity may represent a novel connection. Our findings provide insight into novel aspects of cell wall integrity in this pathogen and lay a foundation for its more detailed analysis.
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影响因子：
7.8
作者：
Delley, P A;Hall, M N
通讯作者：
Hall, M N
影响因子：
--
作者：
Bimbó, A;Jia, YH;Liu, JH
通讯作者：
Liu, JH
机械感应的分子机制及其在真菌接触传感中的作用。
DOI：
10.1038/nrmicro1960
发表时间：
2008-09
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.3
作者：
Bidlingmaier, S;Weiss, EL;Snyder, M
通讯作者：
Snyder, M
DOI：
10.1073/pnas.93.23.13223
发表时间：
1996-11-12
影响因子：
11.1
作者：
Kohler, JR;Fink, GR
通讯作者：
Fink, GR