A 314-bp SINE Insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs
A 314-bp SINE Insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs
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ZNF2 启动子区域中的 314 bp SINE 插入可能充当与猪脂肪沉积调节相关的阻遏蛋白
DOI：
10.1016/j.jia.2022.08.128
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发表时间：
2022-08
影响因子：
4.8
通讯作者：
Song Cheng yi
中科院分区：
文献类型：
--
作者：
Gu Hao;Du Zhanyu;Eduard Murani;Enrico D'Aless;ro;Chen Cai;Wang Xiaoyan;Mao jiu de;Klaus Wimmers;Song Cheng yi
逆转录转座子是一种可以在基因组上调动自身的DNA片段，可以产生遗传变异，并根据插入多态性开发分子标记。锌指蛋白（ZNF）是真核动物中最丰富的蛋白质之一，其功能极其多样，在基因调控中尤其重要。在当前的研究中，进行生物信息学预测来筛选六个ZNF基因（ZNF2、ZNF3、ZNF7、ZNF8、ZNF10和ZNF12）中的逆转录转座子插入多态性（RIP）。这些ZNF中存在6个RIP，包括ZNF2内含子1中的1个短散布核元件（SINE）RIP和内含子3中的1个长散布核元件1（L1）RIP，ZNF3的5'侧翼区中的1个SINE RIP和内含子2中的1个SINE RIP，ZNF7的3'UTR中的1个SINE RIP和内含子2中的1个L1 RIP发现了 ZNF12，并通过 PCR 证实了它们的存在。通过双荧光素酶测定在三种细胞系中研究ZNF2第一个内含子（靠近ZNF2核心启动子）中的SINE RIP对基因活性的影响。我们的结果表明，在ZNF2内含子1中插入SINE，在宫颈癌细胞和猪原代胚胎成纤维细胞（HeLa和PEF）中极显着地抑制核心启动子活性（P<0.01），因此SINE可能起到抑制子的作用。该 SINE RIP 还显着（P<0.05）影响约克夏猪的校正背膘厚度。 ZNF2第一个内含子中插入SINE的个体的校正背膘厚度显着高于未插入SINE的个体(P<0.05)。总之，我们的数据表明，RIPs在这些ZNF基因的遗传变异中发挥着重要作用，ZNF2内含子1中的SINE RIP可能为猪育种中脂肪沉积的筛查提供有用的分子标记。
Retrotransposons, a type of DNA fragment that can mobilize itself on genome, can generate genetic variations and develop for molecular markers based on the insertion polymorphism. Zinc finger proteins (ZNFs) are among the most abundant proteins in eukaryotic animals, and their functions are extraordinarily diverse and particularly important in gene regulation. In the current study, bioinformatic prediction was performed to screen for retrotransposon insertion polymorphisms (RIPs) in sixZNFgenes (ZNF2, ZNF3, ZNF7, ZNF8, ZNF10andZNF12). Six RIPs in theseZNFs, including one short interspersed nuclear element (SINE) RIP in intron 1 and one long interspersed nuclear element 1 (L1) RIP in intron 3 ofZNF2, one SINE RIP in 5′ flanking region and one SINE RIP in intron 2 ofZNF3, one SINE RIP in 3′ UTR ofZNF7and one L1 RIP in intron 2 ofZNF12, were discovered and their presence was confirmed by PCR. The impact of the SINE RIP in the first intron ofZNF2, which is close to the core promoter ofZNF2, on the gene activity was investigated by dual-luciferase assay in three cell lines. Our results showed that the SINE insertion in the intron 1 ofZNF2repressed the core promoter activity extremely significantly (P<0.01) in cervical cancer cells and porcine primary embryonic fibroblasts (HeLa and PEF), thus SINE may act as a repressor. This SINE RIP also significantly (P<0.05) affected the corrected back fat thickness in Yorkshire pigs. The corrected back fat thickness of individuals with SINE insertion in the first intron ofZNF2was significantly (P<0.05) higher than that of individuals without SINE insertion. In summary, our data suggested that RIPs play important roles in the genetic variations of theseZNFgenes and SINE RIP in the intron 1 ofZNF2may provide a useful molecular marker for the screening of fat deposition in the pig breeding.
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影响因子：
64.5
作者：
FEDOROFF, N;WESSLER, S;SHURE, M
通讯作者：
SHURE, M
DOI：
10.1146/annurev-genom-082509-141802
发表时间：
2011
影响因子：
8.7
作者：
Beck CR;Garcia-Perez JL;Badge RM;Moran JV
通讯作者：
Moran JV
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影响因子：
4.9
作者：
Lee J;Mun S;Kim DH;Cho CS;Oh DY;Han K
通讯作者：
Han K
影响因子：
4.4
作者：
T. Hron;H. Fábryová;D. Elleder
通讯作者：
T. Hron;H. Fábryová;D. Elleder
影响因子：
4.8
作者：
Hu Bin;Mo De-lin;Chen Yao-sheng
通讯作者：
Chen Yao-sheng