Genetic characterization and molecular epidemiological analysis of novel enterovirus EV-B80 in China.
Genetic characterization and molecular epidemiological analysis of novel enterovirus EV-B80 in China.
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中国新型肠道病毒EV-B80的遗传特征及分子流行病学分析
DOI：
10.1038/s41426-018-0196-9
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发表时间：
2018-11-28
影响因子：
13.2
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Han Z;Zhang Y;Huang K;Cui H;Hong M;Tang H;Song Y;Yang Q;Zhu S;Yan D;Xu W
肠道病毒B80（EV-B80）是一种新发现的肠道病毒B型血清型。迄今为止，在GenBank中只有两个EV-B80的全长基因组序列，国内和国际上对EV-B80的研究很少。需要更多关于EV-B80的信息和研究来评估其遗传特征，系统发育关系以及与肠道病毒疾病的关联。本研究报告了我国3株新疆EV-B80和1株西藏EV-B80的系统发育特征。4株病毒的基因组全长序列与EV-B80原型病毒的核苷酸同源性为78.8%~ 79%，氨基酸同源性为94%~ 94.2%，显示出进化的趋势。基于VP 1基因的最大似然系统发育树分析表明，EV-B80毒株可能起源于中国大陆，并存在3种基因型（A-C）。重组分析显示，在所有四个EV-B80株的种内重组在非结构区域沿着两个重组模式。由于地理因素的影响，EV-B株的共同进化形成了两种不同的循环模式。在新疆两个地区进行的EV-B80抗体阳性率研究也显示，与EV-B106和EV-B89的其他研究相比，EV-B80在中国新疆广泛流行。所有四个EV-B80菌株都不对温度敏感，显示出较高的群体传播性。总之，本研究报告了EV-B80的全长基因组序列，并为全球EV-B80分子流行病学提供了有价值的信息。
Enterovirus B80 (EV-B80) is a newly identified serotype belonging to the enterovirus B species. To date, only two full-length genomic sequences of EV-B80 are available in GenBank, and few studies on EV-B80 have been conducted in China or worldwide. More information and research on EV-B80 is needed to assess its genetic characteristics, phylogenetic relationships, and association with enteroviral diseases. In this study, we report the phylogenetic characteristics of three Xinjiang EV-B80 strains and one Tibet EV-B80 strain in China. The full-length genomic sequences of four strains show 78.8–79% nucleotide identity and 94–94.2% amino acid identity with the prototype of EV-B80, indicating a tendency for evolution. Based on a maximum likelihood phylogenetic tree based on the entireVP1region, three genotypes (A–C) were defined, revealing the possible origin of EV-B80 strains in the mainland of China. Recombination analysis revealed intraspecies recombinations in all four EV-B80 strains in nonstructural regions along with two recombination patterns. Due to the geographic factor, the coevolution ofEV-Bstrains formed two different patterns of circulation. An antibody seroprevalence study against EV-B80 in two Xinjiang prefectures also showed that EV-B80 strains were widely prevalent in Xinjiang, China, compared to other studies on EV-B106 and EV-B89. All four EV-B80 strains are not temperature sensitive, showing a higher transmissibility in the population. In summary, this study reports the full-length genomic sequences of EV-B80 and provides valuable information on global EV-B80 molecular epidemiology.
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影响因子：
5.4
作者：
Oberste，MS;Maher，K;Pallansch，MA
通讯作者：
Pallansch，MA
影响因子：
5.4
作者：
Oberste, MS;Maher, K;Pallansch, MA
通讯作者：
Pallansch, MA
影响因子：
2.7
作者：
Calderon, Katherine I.;Diaz-de Cerio, Maria;Cabrerizo, Maria
通讯作者：
Cabrerizo, Maria
影响因子：
3.8
作者：
Oberste, MS;Maher, K;Pallansch, MA
通讯作者：
Pallansch, MA
影响因子：
5.3
作者：
Martin DP;Murrell B;Golden M;Khoosal A;Muhire B
通讯作者：
Muhire B