Clinically translatable quantitative molecular photoacoustic imaging with liposome-encapsulated ICG J-aggregates.
Clinically translatable quantitative molecular photoacoustic imaging with liposome-encapsulated ICG J-aggregates.
复制标题
使用脂质体封装的 ICG J 聚集体进行临床可翻译的定量分子光声成像。
DOI：
10.1038/s41467-021-25452-3
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发表时间：
2021-09-13
影响因子：
16.6
通讯作者：
Bouchard RR
中科院分区：
文献类型：
--
作者：
Wood CA;Han S;Kim CS;Wen Y;Sampaio DRT;Harris JT;Homan KA;Swain JL;Emelianov SY;Sood AK;Cook JR;Sokolov KV;Bouchard RR
光声成像是一种功能性和分子成像技术，具有高灵敏度和高时空分辨率。然而，PA成像的广泛使用受到目前可用的造影剂的限制，这些造影剂要么缺乏用于深度成像的PA信号生成能力，要么它们的吸收光谱与血红蛋白重叠，从而降低灵敏度。在这里，我们报告了一种基于载有J-聚集的吲哚菁绿色（ICG）染料（即，PAtrace），我们合成，生物缀合，并表征，以解决这些限制。然后，我们在体模、体外和体内PA成像环境中验证了PAtrace在叶酸受体α阳性卵巢癌模型中的光谱解混准确性和靶向功效。这些研究结果表明，与单体ICG相比，PAtrace同时提供了显著改善的造影剂定量/灵敏度和SO2估计准确度。PAtrace的性能属性和FDA批准的组分组成使其成为未来临床分子PA成像的有前途的试剂。光声成像受到造影剂缺乏的限制，造影剂可以在深度实现分子和生理成像的组合。在这里，作者通过开发和验证基于载有J-聚集的吲哚菁绿色染料的靶向脂质体的造影剂来解决这些限制。
Photoacoustic (PA) imaging is a functional and molecular imaging technique capable of high sensitivity and spatiotemporal resolution at depth. Widespread use of PA imaging, however, is limited by currently available contrast agents, which either lack PA-signal-generation ability for deep imaging or their absorbance spectra overlap with hemoglobin, reducing sensitivity. Here we report on a PA contrast agent based on targeted liposomes loaded with J-aggregated indocyanine green (ICG) dye (i.e., PAtrace) that we synthesized, bioconjugated, and characterized to addresses these limitations. We then validated PAtrace in phantom, in vitro, and in vivo PA imaging environments for both spectral unmixing accuracy and targeting efficacy in a folate receptor alpha-positive ovarian cancer model. These study results show that PAtrace concurrently provides significantly improved contrast-agent quantification/sensitivity and SO2 estimation accuracy compared to monomeric ICG. PAtrace’s performance attributes and composition of FDA-approved components make it a promising agent for future clinical molecular PA imaging. Photoacoustic imaging is limited by a lack of contrast agents which can enable combined molecular and physiological imaging at depth. Here the authors address these limitations by developing and validating a contrast agent based on targeted liposomes loaded with J-aggregated indocyanine green dye.
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影响因子：
--
作者：
Dehghani, Hamid;Eames, Matthew E.;Yalavarthy, Phaneendra K.;Davis, Scott C.;Srinivasan, Subhadra;Carpenter, Colin M.;Pogue, Brian W.;Paulsen, Keith D.
通讯作者：
Paulsen, Keith D.
模拟图像声和超声成像的组织幻象。
影响因子：
3.4
作者：
Cook JR;Bouchard RR;Emelianov SY
通讯作者：
Emelianov SY
DOI：
10.1158/1078-0432.ccr-15-0314
发表时间：
2015-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Kimbrough CW;Khanal A;Zeiderman M;Khanal BR;Burton NC;McMasters KM;Vickers SM;Grizzle WE;McNally LR
通讯作者：
McNally LR
影响因子：
5.4
作者：
Bulbake U;Doppalapudi S;Kommineni N;Khan W
通讯作者：
Khan W
影响因子：
5.9
作者：
Ermolayev, Vladimir;Dean-Ben, Xose Luis;Razansky, Daniel
通讯作者：
Razansky, Daniel