A survey of carbonic anhydrase mRNA expression in enamel cells.
A survey of carbonic anhydrase mRNA expression in enamel cells.
复制标题
釉质细胞中碳酸酐酶 mRNA 表达的调查。
DOI：
10.1016/j.bbrc.2010.02.116
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发表时间：
2010-03-19
影响因子：
3.1
通讯作者：
Paine, Michael L.
中科院分区：
文献类型：
--
作者：
Lacruz, Rodrigo S.;Hilvo, Mika;Kurtz, Ira;Paine, Michael L.
牙釉质的形成需要在发育的各个阶段严格控制 pH 稳态，以防止晶体生长受到干扰。碳酸酐酶 (CA) 加速碳酸氢盐的生成被认为是成釉细胞用来调节细胞外 pH 值的途径之一，但迄今为止在牙釉质形成过程中仅报道了两种同工酶（CA II 和 CA VI）。哺乳动物 CA 家族包含 16 种不同的亚型，其中 13 种具有酶活性。我们通过 RT-PCR 对新生小鼠釉质器官上皮 (EOE) 细胞、7 日龄动物分泌性成釉细胞和 LS8 成釉细胞系中所有已知 CA 亚型的表达进行了系统筛选。结果表明，所有 CA 亚型均由体内 EOE/成釉细胞表达。表达最高的是催化同工酶 CA II、VI、IX 和 XIII，以及无催化同工酶 CA XI 亚型。 1 天的 EOE 细胞和 7 天的分泌期成釉细胞之间的 CA 表达水平仅存在微小差异，而 LS8 细胞表达的 CA 亚型比这两种细胞少。本文报道的成釉细胞广泛表达 CA 有助于我们了解牙釉质发育过程中的 pH 稳态，并证明其复杂性。我们的结果还强调了 pH 值调节在牙釉质发育过程中发挥的关键作用。
Enamel formation requires rigid control of pH homeostasis during all stages of development to prevent disruptions to crystal growth. The acceleration of the generation of bicarbonate by carbonic anhydrases (CA) has been suggested as one of the pathways used by ameloblasts cells to regulate extracellular pH yet only two isozymes (CA II and CA VI) have been reported to date during enamel formation. The mammalian CA family contains 16 different isoforms of which 13 are enzymatically active. We have conducted a systematic screening by RT-PCR on the expression of all known CA isoforms in mouse enamel organ epithelium (EOE) cells dissected from new born, in secretory ameloblasts derived from 7-day old animals, and in the LS8 ameloblast cell line. Results show that all CA isoforms are expressed by EOE/ameloblast cells in vivo. The most highly expressed are the catalytic isozymes CA II, VI, IX, and XIII, and the acatalytic CA XI isoform. Only minor differences were found in CA expression levels between 1-day EOE cells and 7-day old secretory stage ameloblasts, whereas LS8 cells expressed fewer CA isoforms than both of these. The broad expression of CAs by ameloblasts reported here contributes to our understanding of pH homeostasis during enamel development and demonstrates its complexity. Our results also highlight the critical role that regulation of pH plays during the development of enamel.
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影响因子：
--
作者：
Pan, Pei-wen;Rodriguez, Alejandra;Parkkila, Seppo
通讯作者：
Parkkila, Seppo
影响因子：
5.5
作者：
Pan, PW;Leppilampi, M;Parkkila, S
通讯作者：
Parkkila, S
影响因子：
4.2
作者：
Lacruz, Rodrigo S.;Nanci, Antonio;Kurtz, Ira;Wright, J. Timothy;Paine, Michael L.
通讯作者：
Paine, Michael L.
影响因子：
3
作者：
CHEN, LS;COUWENHOVEN, RI;SNEAD, ML
通讯作者：
SNEAD, ML
影响因子：
4.2
作者：
Leinonen, J;Kivelä, J;Rajaniemi, H
通讯作者：
Rajaniemi, H