Differential expression of oestrogen receptor alpha following reproductive experience in young and middle-aged female rats.
Differential expression of oestrogen receptor alpha following reproductive experience in young and middle-aged female rats.
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DOI：
10.1111/j.1365-2826.2009.01874.x
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发表时间：
2009-06
影响因子：
3.2
通讯作者：
Bridges RS
中科院分区：
文献类型：
--
作者：
Byrnes EM;Babb JA;Bridges RS
生殖经验（即怀孕和哺乳）改变了许多生理和行为终点，其中许多与生殖功能有关，并受雌激素调节。例如，生殖经验显着减弱雌激素诱导的催乳素激增的下午，动情前期和循环雌二醇水平降低在这个时候。虽然在垂体前叶中观察到对雌激素受体α（ERα）的产次相关影响，但目前尚无关于产次诱导的脑中ERα变化的数据。因此，本研究的目的是检查产次对生殖相关脑区ERα表达的影响。此外，由于先前的研究结果表明，生殖经验的长期影响通常是发情周期依赖性的，因此在发情周期的两个阶段（发情间期和发情前期）检查ERα。最后，由于ERα的表达受年龄的显著影响，因此本研究包括青年和中年女性。在年龄匹配、周期性经产（一次妊娠和哺乳）和未经产雌性动物以及年龄匹配、非周期性（持续发情）12月龄经产和未经产雌性动物中，使用免疫组织化学法测定参与生殖行为调节的选定脑区的ERα状态。在视前内侧区和杏仁核内侧区观察到ERα细胞数量的显著变化，这是生殖经验的结果，具有雌激素依赖性。这些发现表明，ERα活性的显着变化发生在大脑中作为生殖经验的功能。
Reproductive experience (i.e. pregnancy and lactation) alters a number of physiological and behavioral endpoints, many of which are related to reproductive function and are regulated by oestrogen. For example, reproductive experience significantly attenuates the oestradiol-induced prolactin surge on the afternoon of proestrous and circulating oestradiol levels are reduced at this time. While parity-related effects on oestrogen receptor alpha (ERα) have been observed within the anterior pituitary, there are currently no data regarding possible parity-induced alterations in ERα in the brain. Thus, the purpose of the current study was to examine the effect of parity on the expression of ERα in reproductively relevant brain regions. Moreover, because previous findings have demonstrated that the long-term effects of reproductive experience are often oestrous cycle dependent, ERα was examined at two stages of the oestrous cycle (diestrous and proestrous). Finally, as the expression of ERα is significantly influenced by age, both young and middle-aged females were included in the present study. ERα status was determined using immunohistochemistry in select brain regions involved in the regulation of reproductive behavior in age-matched, cycling primiparous (one pregnancy and lactation) and nulliparous females as well as in age-matched, non-cycling (persistent estrus) 12 month-old primiparous and nulliparous females. Significant shifts in ERα cell numbers were observed in the medial preoptic area and medial amygdala as a consequence of reproductive experience in an oestrous-dependent manner. These findings indicate that significant changes in ERα activity occur in the brain as a function of reproductive experience.
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影响因子：
3.2
作者：
Byrnes, E. M.;Bridges, R. S.
通讯作者：
Bridges, R. S.
影响因子：
3.8
作者：
Gatewood, JD;Morgan, MD;Kinsley, CH
通讯作者：
Kinsley, CH
影响因子：
2.9
作者：
Hosokawa, Nami;Chiba, Atsuhiko
通讯作者：
Chiba, Atsuhiko
影响因子：
3.7
作者：
Hou, YP;Yang, SP;Voogt, JL
通讯作者：
Voogt, JL
影响因子：
2.9
作者：
MANN, PE;BRIDGES, RS
通讯作者：
BRIDGES, RS