Isolation and characterization of buffalo (bubalus bubalis) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy.
Isolation and characterization of buffalo (bubalus bubalis) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy.
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源自妊娠早期羊膜的水牛 (bubalus bubalis) 羊膜间充质干细胞的分离和表征
DOI：
10.1292/jvms.17-0556
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发表时间：
2018-04-27
期刊：
影响因子：
--
通讯作者：
Shi D
中科院分区：
文献类型：
--
作者：
Deng Y;Huang G;Zou L;Nong T;Yang X;Cui J;Wei Y;Yang S;Shi D
家畜羊膜间充质干细胞(AMSCs)是动物繁殖和兽医治疗的宝贵资源。本研究旨在探索一种适合水牛AMSCs分离培养的方法，并对其生物学特性进行鉴定。胰酶-EDTA和I型胶原酶联合消化比单独使用胰酶-EDTA或I型胶原酶更能有效地获得纯净的bAMSCs。体外培养的BAMSCs生长稳定，呈成纤维细胞样形态，呈漩涡状集落。骨髓间充质干细胞特异性标志物CD44、CD90、CD105、CD73、β整合素(CD29)、CD166阳性，多能标志物OCT4、SOX2、NANOG、REX-1、SSEA-1、SSEA-4、TRA-1-81阳性，而造血系标志物CD34、CD45、上皮细胞特异性标志物细胞角蛋白18阴性。糖原合成酶-3抑制剂：坎帕隆促进bAMSCs向神经系分化。本研究为获得和鉴定bAMSCs提供了一种有效的方法，该细胞已被证明是水牛细胞重编程研究和转基因动物生产的有用细胞资源。
Amniotic mesenchymal stem cells (AMSCs) from livestock are valuable resources for animal reproduction and veterinary therapeutic. The purpose of this study is to explore a suitable way to isolate and culture the buffalo AMSCs (bAMSCs), and to identify their biological characteristics. Digestion with a combination of trypsin-EDTA and collagenase type I could obtain pure bAMSCs more effectively than trypsin-EDTA or collagenase type I alone. bAMSCs could proliferate steadily in vitro culture and exhibited fibroblastic-like morphology in vortex-shaped colony. bAMSCs were positive for MSC-specific markers CD44, CD90, CD105, CD73, β-integrin (CD29) and CD166, and pluripotent markers OCT4, SOX2, NANOG, REX-1, SSEA-1, SSEA-4 and TRA-1-81, but negative for hematopoietic markers CD34, CD45 and epithelial cells specific marker Cytokeratin 18. In addition, bAMSCs were capable of differentiating into adipogenic, osteogenic, chondrogenic and neural lineages, with expression of FABP4, Ost, ACAN, COL2A1, Nestin and β III-tubulin. Glycogen synthase kinase 3 inhibitor: kenpaullone promoted bAMSCs to differentiate into neural lineage. This study provides an effective protocol to obtain and characterize bAMSCs, which have proven useful as a cell resource for buffalo cell reprogramming studies and transgenic animal production.
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DOI：
10.1007/s11626-015-9920-0
发表时间：
2015-10-01
影响因子：
2.1
作者：
Ghosh, Kaushalya;Kumar, Rajesh;Yadav, P. S.
通讯作者：
Yadav, P. S.
影响因子：
2.2
作者：
Mann, A.;Yadav, R. P.;Yadav, P. S.
通讯作者：
Yadav, P. S.
DOI：
10.1111/j.1365-2184.1987.tb01309.x
发表时间：
1987-05-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;GERASIMOV, UV
通讯作者：
GERASIMOV, UV
影响因子：
5.2
作者：
in't Anker, PS;Scherjon, SA;Kanhai, HHH
通讯作者：
Kanhai, HHH
影响因子：
5.3
作者：
Jin, KL;Mao, XO;Greenberg, DA
通讯作者：
Greenberg, DA