Total flavonoids extracted from Nervilia Fordii function in polycystic ovary syndrome through IL-6 mediated JAK2/STAT3 signaling pathway
Total flavonoids extracted from Nervilia Fordii function in polycystic ovary syndrome through IL-6 mediated JAK2/STAT3 signaling pathway
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芥花总黄酮通过 IL-6 介导的 JAK2/STAT3 信号通路发挥治疗多囊卵巢综合征的作用
DOI：
10.1042/bsr20181380
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发表时间：
2018-11
影响因子：
4
通讯作者：
Jiale Song
中科院分区：
文献类型：
--
作者：
Yanyuan Zhou;Liang Lv;Qinghua Liu;Jiale Song
大剂量的黄酮类化合物可以治愈许多疾病，而且没有严重的副作用。然而，黄酮类化合物在治疗多囊卵巢综合征(PCOS)中的作用尚未见报道。因此，选择从芥花中提取的总黄酮来探讨其对多囊卵巢综合症的治疗效果。构建PCOS大鼠模型，探讨总黄酮治疗PCOS的作用。采用ELISA法评估总黄酮联合或不联合外源白细胞介素6(IL-6)处理下排卵功能的变化。Western blot、实时荧光定量PCR和免疫组化分析相关分子机制。我们发现总黄酮通过部分抑制JAK2/STAT3通路的激活，部分上调IL-6的表达，部分下调细胞因子信号传导抑制因子3（SOCS3）的表达，显着升高PCOS-IR大鼠血清促卵泡激素（FSH）水平，并急剧降低血清黄体生成素（LH）、睾酮（T）和胰岛素（INS）水平。 PCOS 大鼠的卵巢。在PCOS大鼠模型中，总黄酮对动情周期、血清FSH、LH、T和INS水平的影响被IL-6部分减弱。此外，IL-6显着逆转总黄酮对PCOS大鼠卵巢JAK2/STAT3磷酸化、IL-6和SOCS3表达的影响。从青花中提取的总黄酮可能会诱导卵巢中IL-6的表达，并可作为治疗PCOS的潜在治疗药物。
Large doses of flavonoids could cure many diseases with no serious side effects. However,.the role of flavonoids in the treatment of polycystic ovary syndrome (PCOS) has not been.reported. Therefore, total flavonoids extracted from Nervilia Fordii were selected to explore.its therapeutic efficiency in PCOS. PCOS rat model was constructed to explore the role of.total flavonoids in the treatment of PCOS. ELISA was used to assess the changes of ovulation.function under the treatment of total flavonoids with or without exogenous interleukin-6.(IL-6).Western blot, real-time PCR and immunohistochemistry were carried out to assess the.related molecular mechanisms. We explored that total flavonoids obviously increased the.serum levels of follicle-stimulating hormone (FSH), and sharply decreased the serum levels.of luteinizing hormone (LH), testosterone (T) and insulin (INS) in the PCOS-IR rats via partly.inhibiting the activation of JAK2/STAT3 pathway, partially up-regulating the IL-6 expression.and partially down-regulating the suppressor of cytokine signaling 3 (SOCS3) expression.in ovaries of PCOS rats. The effect of total flavonoids on estrous cycles, serum levels of.FSH, LH, T and INS were partially attenuated by IL-6 in PCOS rat model. Moreover, IL-6.significantly reversed the effect of total flavonoids on the phosphorylation of JAK2/STAT3,.the expression of IL-6 and SOCS3 in ovaries of PCOS rats. Total flavonoids extracted from.Nervilia Fordii might induce the expression of IL-6 in ovary and act as a potential therapeutic.drug for the treatment of PCOS.
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影响因子：
1.8
作者：
Wiweko B;Indra I;Susanto C;Natadisastra M;Hestiantoro A
通讯作者：
Hestiantoro A
影响因子：
2.7
作者：
Yuanyuan Wu;Peng-fen Li;Dan Zhang;Ying-pu Sun
通讯作者：
Yuanyuan Wu;Peng-fen Li;Dan Zhang;Ying-pu Sun
影响因子：
7.1
作者：
Williamson, G;Manach, C
通讯作者：
Manach, C
DOI：
--
发表时间：
1973-06
期刊：
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
影响因子：
--
作者：
Shizhen Li;F. Smith;G. Stuart
通讯作者：
Shizhen Li;F. Smith;G. Stuart
影响因子：
5.8
作者：
Glintborg, Dorte;Rubin, Katrine Hass;Andersen, Marianne
通讯作者：
Andersen, Marianne