Enriched environment protects the nigrostriatal dopaminergic system and induces astroglial reaction in the 6-OHDA rat model of Parkinson's disease.
Enriched environment protects the nigrostriatal dopaminergic system and induces astroglial reaction in the 6-OHDA rat model of Parkinson's disease.
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DOI：
10.1111/j.1471-4159.2009.06001.x
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发表时间：
2009-05
影响因子：
4.7
通讯作者：
Mascó DH
中科院分区：
文献类型：
--
作者：
Anastasía A;Torre L;de Erausquin GA;Mascó DH
丰富的环境（EE）在几种神经退行性疾病的动物模型中具有神经保护作用。它刺激营养因子的表达并改变星形胶质细胞群，据说星形胶质细胞群具有神经保护作用。我们研究了EE对6-羟基多巴胺（6-OHDA）诱导的神经元死亡后，单侧给药的内侧前脑束，达到85-95%的多巴胺能神经元在黑质3周后的影响。在6-OHDA注射前后连续暴露于EE 3周可预防神经元死亡（通过酪氨酸羟化酶染色评估），保护黑质纹状体通路（通过荧光金逆行标记评估）并减少运动损伤。注射6-OHDA后四天，EE与胶质纤维酸性蛋白染色显着增加相关，并阻止了神经元死亡（通过Fluoro Jade-B评估），但并未导致前黑质中酪氨酸羟化酶染色的部分丧失。这些结果有力地表明，EE保留了整个黑质纹状体系统对6-OHDA诱导的毒性，并表明，早期损伤后星形胶质细胞反应可能参与神经保护机制。
Enriched environment (EE) is neuroprotective in several animal models of neurodegeneration. It stimulates the expression of trophic factors and modifies the astrocyte cell population which has been said to exert neuroprotective effects. We have investigated the effects of EE on 6-hydroxydopamine (6-OHDA)-induced neuronal death after unilateral administration to the medial forebrain bundle, which reaches 85–95% of dopaminergic neurons in the substantia nigra after 3 weeks. Continuous exposure to EE 3 weeks before and after 6-OHDA injection prevents neuronal death (assessed by tyrosine hydroxylase staining), protects the nigrostriatal pathway (assessed by Fluorogold retrograde labeling) and reduces motor impairment. Four days after 6-OHDA injection, EE was associated with a marked increase in glial fibrillary acidic protein staining and prevented neuronal death (assessed by Fluoro Jade-B) but not partial loss of tyrosine hydroxylase staining in the anterior substantia nigra. These results robustly demonstrate that EE preserves the entire nigrostriatal system against 6-OHDA-induced toxicity, and suggests that an early post-lesion astrocytic reaction may participate in the neuro-protective mechanism.
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影响因子：
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