Arrangement of kinetochore proteins and satellite DNA in neuronal interphase nuclei: changes induced by gamma-aminobutyric acid (GABA).
Arrangement of kinetochore proteins and satellite DNA in neuronal interphase nuclei: changes induced by gamma-aminobutyric acid (GABA).
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神经元间期核中动粒蛋白和卫星 DNA 的排列：γ-氨基丁酸 (GABA) 诱导的变化。
DOI：
10.1016/0014-4827(91)90476-b
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发表时间：
1991
影响因子：
3.7
通讯作者：
U. De Boni
中科院分区：
文献类型：
--
作者：
T. Holowacz;U. De Boni
离散的染色质结构域在神经元间期核中占据特定的核隔室。核旋转，定义为间期染色质结构域的运动，已被提出在特定的染色质结构域的位置，以核隔室，这是适合于细胞的生理状态或分化状态的功能。这种染色质运动的速率可能会被包括γ-氨基丁酸（GABA）在内的试剂增加，这可能会导致基因表达的变化。为了测试GABA是否也会引起特定染色质结构域的空间重排，通过免疫荧光法测定小鼠背根神经节神经元核中动粒的三维分布。此外，着丝粒卫星DNA序列定位原位杂交使用生物素标记的小鼠卫星DNA探针，然后免疫荧光。着丝粒成簇出现，与核仁或在中间核质区，核仁和核膜之间。卫星DNA序列的集群被发现与核仁或整个核质。值得注意的是，核质动粒始终占据距离核中心5 μm的距离，占球形核半径的70%。暴露于GABA的神经元诱导的着丝粒，这可能代表染色体的运动交替核隔室，以适应一个新的转录状态的显着重组。
Discrete chromatin domains occupy specific nuclear compartments in neuronal interphase nuclei. Nuclear rotation, defined as the motion of interphase chromatin domains, has been proposed to function in the placement of specific chromatin domains to nuclear compartments which are appropriate to the physiological state or the state of differentiation of the cell. Rates of this chromatin motion may be increased by agents, including gamma-amino butyric acid (GABA), which may cause changes in gene expression. To test whether GABA also causes a spatial rearrangement of specific chromatin domains, the three-dimensional distribution of kinetochores in nuclei of mouse dorsal root ganglion neurons was determined by immunofluorescence. In addition, centromeric satellite DNA sequences were localized byin situhybridization using a biotinylated mouse satellite DNA probe followed by immunofluorescence. Kinetochores occurred in clusters, associated with nucleoli or in intermediate nucleoplasmic regions, between the nucleolus and the nuclear membrane. Clusters of satellite DNA sequences were found either associated with nucleoli or throughout the nucleoplasm. Strikingly, nucleoplasmic kinetochores consistently occupied a 5-μm distance from the nuclear center, representing 70% of the spherical nuclear radius. Exposure of neurons to GABA induced a significant reorganization of kinetochores which may represent movement of chromosomes to alternate nuclear compartments to accomodate a new transcriptional state.
影响因子：
3.7
作者：
PADDOCK, SW;ALBRECHTBUEHLER, G
通讯作者：
ALBRECHTBUEHLER, G
影响因子：
56.9
作者：
BORDEN, J;MANUELIDIS, L
通讯作者：
MANUELIDIS, L
影响因子：
56.9
作者：
GREENBERG, ME;ZIFF, EB;GREENE, LA
通讯作者：
GREENE, LA