A Two-Step Protocol to Remove Endotoxins from Human-Like Collagen
A Two-Step Protocol to Remove Endotoxins from Human-Like Collagen
复制标题
从类人胶原蛋白中去除内毒素的两步方案
DOI：
10.1080/01496395.2014.978467
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发表时间：
2015-01
影响因子：
2.8
通讯作者：
Xue, Wenjiao
中科院分区：
文献类型：
--
作者：
Fan, Daidi;Ma, Xiaoxuan;Mi, Yu;Xue, Wenjiao
研究了一种从重组类人胶原中去除内毒素的两步法。为满足临床需要，首先采用Triton X-114两相萃取系统去除大量的内毒素，然后采用亲和层析进行进一步纯化。在响应面法优化的最佳实验条件下，内毒素水平为0.025˜0.25EU/mg，蛋白质回收率为81.9%，内毒素清除率为99%，蛋白质回收率较高。因此，两步法可以作为生物技术过程中消除内毒素的一种有效方法。
A two-step protocol to remove endotoxins from recombinant human-like collagen was investigated. To meet the clinical need, Triton X-114 two-phase extraction system was applied as the first step to remove large amounts of endotoxins, while affinity chromatography was used as the second step to achieve further purification. Under the best experimental condition optimized through response surface methodology techniques, endotoxin level was 0.025˜0.25 EU/mg and protein recovery was 81.9%, respectively, which indicated that 99% of endotoxins were eliminated with high protein recovery. Therefore, the two-step protocol can be exploited as an efficient method for endotoxin elimination in biotechnology processes.
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影响因子：
--
作者：
Liping Li;R. G. Luo
通讯作者：
Liping Li;R. G. Luo
DOI：
--
发表时间：
2002
期刊：
Journal of Chemical Industry and Engineering
影响因子：
--
作者：
Jirong Song
通讯作者：
Jirong Song
影响因子：
5
作者：
A. Lopes;V. Santos-Ebinuma;Letícia Celia de Lencastre Novaes;J. Molino;Leandro R. S. Barbosa;A. Pessoa;C. O. Rangel-Yagui
通讯作者：
A. Lopes;V. Santos-Ebinuma;Letícia Celia de Lencastre Novaes;J. Molino;Leandro R. S. Barbosa;A. Pessoa;C. O. Rangel-Yagui
DOI：
10.1016/j.msec.2014.07.058
发表时间：
2014-10-01
影响因子：
7.9
作者：
Zhang, Jingjing;Ma, Xiaoxuan;Ma, Pei
通讯作者：
Ma, Pei
影响因子：
--
作者：
N. Zhang;Z. Qi;Huiguo Duan;Yuhua Xie;Jian Yu;Changxu Lu;Xunli Liu
通讯作者：
N. Zhang;Z. Qi;Huiguo Duan;Yuhua Xie;Jian Yu;Changxu Lu;Xunli Liu