Vital Functions Contribute to the Spread of Extracellular Fluids in the Brain: Comparison Between Life and Death
Vital Functions Contribute to the Spread of Extracellular Fluids in the Brain: Comparison Between Life and Death
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重要功能有助于细胞外液在大脑中的传播：生与死的比较
DOI：
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发表时间：
2020
影响因子：
4.8
通讯作者：
I. Bechmann
中科院分区：
文献类型：
--
作者：
Alina Piotrowska;K. Winter;R. Carare;I. Bechmann
据报道，血管搏动、血管平滑肌细胞的收缩和呼吸可以促进脑间质和脑脊液的运动和清除。本研究的目的是估计这些重要功能的贡献。我们比较了注射后 30 分钟和 90 分钟注射的亲水性示踪剂（Fluoro-Emerald，一种 10 kDa 荧光素偶联葡聚糖胺）在活体麻醉和处死大鼠大脑中的扩散情况。为了确定示踪剂分布的总体模式，我们创建了整个大脑水平横切面的 3D 重建。用层粘连蛋白和 IV 型胶原进行免疫荧光染色以确定示踪剂相对于脑血管基底膜的分布模式。我们发现，在处死大鼠中，扩散广泛局限于脑室周围区域，没有扩散到对侧半球，而在活体麻醉动物中，大量血流沿着脉管系统发生，并在注射后 30 分钟到达表面和对侧半球。正如之前报道的，示踪剂似乎沿着血管基底膜和沿着纤维束定位。因此，我们的数据表明，重要功能对于脑实质内细胞外液的远程运动至关重要。
Vascular pulsations, contractions of vascular smooth muscle cells and breathing have been reported to foster movement and clearance of interstitial and cerebrospinal fluids from the brain. The aim of this study was to estimate the contribution of these vital functions. We compared the spread of an injected hydrophilic tracer (Fluoro-Emerald, a 10 kDa fluorescein-coupled dextran amine) in the brains of live anesthetized and sacrificed rats at 30 and 90 min after injection. To determine the overall pattern of distribution of tracers, we created 3D-reconstructions of the horizontal transections of the whole brain. Immunofluorescence staining with laminin and collagen IV was performed to determine the pattern of distribution of tracer in relation to the cerebrovascular basement membranes. We found that diffusion was widely restricted to the periventricular region in sacrificed rats with no spread to the contralateral hemisphere, while the bulk flow occurred along the vasculature and reached the surface and the contralateral hemisphere as soon as 30 min after injection in live anesthetized animals. The tracer appeared to be localized along the vascular basement membranes and along fiber tracts as reported previously. Thus, our data indicate that vital functions are essential for the remote movement of extracellular fluids within the cerebral parenchyma.
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影响因子：
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作者：
SZENTISTVANYI, I;PATLAK, CS;CSERR, HF
通讯作者：
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影响因子：
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