Effect of botulinum toxin type a on a rat surgical wound model.
Effect of botulinum toxin type a on a rat surgical wound model.
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DOI：
10.3342/ceo.2009.2.1.20
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发表时间：
2009-03
影响因子：
3
通讯作者：
Kim HW
中科院分区：
文献类型：
--
作者：
Lee BJ;Jeong JH;Wang SG;Lee JC;Goh EK;Kim HW
伤口上的张力是决定纤维化和瘢痕形成程度的重要因素之一。我们假设局部A型肉毒杆菌毒素（Botox）诱导的皮肤缺损手术伤口下的肌肉组织麻痹将使手术伤口边缘上的重复张力最小化，这将导致伤口的纤维增生反应和纤维化减少。这是一项前瞻性随机实验研究。在15只成年大鼠的背部分别制作两个不同的手术伤口。两个伤口中的一个注射肉毒杆菌素，另一个伤口用作对照，并且对所有大鼠的伤口都这样做。观察创面大小、纤维化程度、炎症程度、血管增生程度、创面厚度及转化生长因子β1（TGF-β1）的表达。术后3、4周，肉毒杆菌毒素组创面面积与对照组比较差异有统计学意义（P<0.05）。第2周时，Botox组的炎症细胞浸润明显少于对照组（P<0.05）。第4周时，Botox组成纤维细胞数量和纤维化程度均低于对照组（P<0.05）。第8周时，Botox组胶原密度明显高于对照组（P<0.05）。免疫组化染色结果显示，第4周Botox组转化生长因子β1（TGF-β1）表达低于对照组（P<0.05）。与对照组相比，Botox治疗组创面面积较大，炎性细胞浸润和纤维化程度较轻，胶原含量明显增加，TGF-β1表达较低。肉毒杆菌毒素可用于减少手术伤口的纤维化，而不会在需要减少纤维化的情况下损害上皮生长，例如用于治疗喉、气管和鼻狭窄。
The tension on a wound is one of the important factors that determine the degree of fibrosis and scar formation. We hypothesized that local botulinum toxin type A (Botox) induced paralysis of the musculature subjacent to a surgical wound with a skin defect would minimize the repetitive tensile forces on the surgical wound's edges, and this will result in a decreased fibroplastic response and fibrosis of the wound. This is a prospective randomized experimental study. Two distinct surgical wounds were made to the dorsum of 15 adult rats, respectively. One of the 2 wounds was injected with Botox, and the other wound was used as a control, and this was done for all the rats' wounds. We evaluated the wound size, the degree of fibrosis and inflammation, the blood vessel proliferation, the thickness of the wound and the expression of transforming growth factor (TGF)-β1 in the wounds. There were significant differences of wound size at the 3rd and 4th week between the Botox and control groups (P<0.05). The Botox group showed less infiltration of inflammatory cells than the control group at the 2nd week (P<0.05). The Botox group showed a smaller number of fibroblasts and less fibrosis than the control group at the 4th week (P<0.05). The Botox group showed much strong collagen density than the control group at the 8th week (P<0.05). For the immunohistochemical staining, there was a lower transforming growth factor (TGF)-β1 expression in the Botox group than that of the control group at the 4th week (P<0.05). The wounds of the Botox-treated group showed a larger wound size, less infiltration of inflammatory cells and less fibrosis, a much greater amount of collagen and a lower expression of TGF-β1 than did the control group. Botox might be used to decrease the fibrosis of a surgical wound without damaging the epithelial growth in situations for which decreased fibrosis is necessary, such as for treating laryngeal, tracheal and nasal stenosis.
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DOI：
10.1097/00002341-200003000-00006
发表时间：
2000-03-01
影响因子：
2
作者：
Hu, D;Sires, BS;Oda, D
通讯作者：
Oda, D
影响因子：
8.9
作者：
Gassner, Holger G.;Brissett, Anthony E.;Sherris, David A.
通讯作者：
Sherris, David A.
影响因子：
3.6
作者：
Gassner, HG;Sherris, DA;Otley, CC
通讯作者：
Otley, CC
影响因子：
2.6
作者：
Blitzer, A;Sulica, L
通讯作者：
Sulica, L
影响因子：
--
作者：
Manuskiatti, W;Fitzpatrick, RE
通讯作者：
Fitzpatrick, RE