5-Aminosalicylic Acid Alters the Gut Bacterial Microbiota in Patients With Ulcerative Colitis.
5-Aminosalicylic Acid Alters the Gut Bacterial Microbiota in Patients With Ulcerative Colitis.
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5-氨基水杨酸改变溃疡性结肠炎患者的肠道细菌微生物群
DOI：
10.3389/fmicb.2018.01274
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发表时间：
2018
影响因子：
5.2
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xu J;Chen N;Wu Z;Song Y;Zhang Y;Wu N;Zhang F;Ren X;Liu Y
背景：本研究旨在阐明5 - 氨基水杨酸（5 - ASA）治疗对溃疡性结肠炎（UC）患者肠道细菌微生物群的影响。
方法：共招募57例UC患者，其中20例未接受治疗，37例接受5 - ASA治疗，组成探索队列。我们通过内镜从所有患者身上采集非炎症和炎症黏膜样本，并使用16S rDNA测序比较肠道细菌谱。随后，10例未接受治疗的UC患者接受5 - ASA治疗，并纳入独立验证研究以确认所得数据。
结果：在未接受治疗的UC患者中，与非炎症黏膜相比，炎症黏膜中的厚壁菌门（如肠球菌）减少，变形菌门（如埃希氏菌 - 志贺氏菌）增加。与未接受治疗的UC患者的炎症黏膜相比，接受5 - ASA治疗的UC患者炎症黏膜中的厚壁菌门（如肠球菌）丰度更高，变形菌门（埃希氏菌 - 志贺氏菌）丰度更低。在验证队列中，给予5 - ASA后，细菌变化与这些数据一致。此外，炎症黏膜中埃希氏菌 - 志贺氏菌与厚壁菌门细菌属之间存在偏态负相关。5 - ASA治疗降低了细菌相关性的强度，并削弱了这种偏态负相关模式。
结论：微生物群失调（主要特征为埃希氏菌 - 志贺氏菌属丰度增加）以及埃希氏菌 - 志贺氏菌与厚壁菌门细菌属之间的偏态负相关是UC患者炎症黏膜的两个特征。5 - ASA治疗可减少埃希氏菌 - 志贺氏菌并削弱偏态相关性，这可能与其治疗效果有关。
Background: The aim of this study was to clarify the effect of 5-aminosalicylic acid (5-ASA) treatment on gut bacterial microbiota in patients with ulcerative colitis (UC). Methods: A total of 57 UC patients, including 20 untreated and 37 5-ASA-treated, were recruited into an exploration cohort. We endoscopically collected both non-inflamed and inflamed mucosal samples from all patients, and compared the gut bacterial profiles using 16S rDNA sequencing. Ten untreated UC patients were then treated with 5-ASA and subsequently recruited for an independent validation study to confirm the acquired data. Results: In untreated UC patients, compared with those in non-inflamed mucosae, Firmicutes (such as Enterococcus) were decreased and Proteobacteria (e.g., Escherichia–Shigella) were increased in the inflamed mucosae. Compared with the inflamed mucosae of untreated UC patients, there was a higher abundance of Firmicutes (e.g., Enterococcus) and lower Proteobacteria (Escherichia–Shigella) in the inflamed mucosae of 5-ASA treated UC patients. In the validation cohort, after administration of 5-ASA, bacterial alteration was consistent with these data. Furthermore, there was a skewed negative correlation between Escherichia–Shigella and bacterial genera of Firmicutes in the inflamed mucosae. 5-ASA treatment decreased the strength of bacterial correlation and weakened the skewed negative correlation pattern. Conclusion: The microbial dysbiosis (mainly characterized by an increased abundance in the Escherichia–Shigella genus) and the skewed negative correlation between Escherichia–Shigella and bacterial genera of Firmicutes are two characteristics of the inflamed mucosae of UC patients. 5-ASA treatment decreases Escherichia–Shigella and weakens the skewed correlations, which may be related to its treatment efficiency.
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影响因子：
11
作者：
de Carcer, Daniel Aguirre;Cuiv, Paraic O.;Morrison, Mark
通讯作者：
Morrison, Mark
DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
2.2
作者：
Costa, M. C.;Silva, G.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
3.7
作者：
Gu S;Chen D;Zhang JN;Lv X;Wang K;Duan LP;Nie Y;Wu XL
通讯作者：
Wu XL
影响因子：
7.6
作者：
Andrews, C. N.;Griffiths, T. A.;Rioux, K. P.
通讯作者：
Rioux, K. P.