Comparative genomics reveals evidence of marine adaptation in Salinispora species.
Comparative genomics reveals evidence of marine adaptation in Salinispora species.
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DOI：
10.1186/1471-2164-13-86
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发表时间：
2012-03-08
期刊：
影响因子：
4.4
通讯作者：
Jensen PR
中科院分区：
文献类型：
--
作者：
Penn K;Jensen PR
放线杆菌代表了大多数海洋细菌群落的一致组成部分，但对这些革兰氏阳性细菌适应海洋环境中的生命的机制知之甚少。在这里，我们使用系统基因组学方法来鉴定海洋放线菌中的海洋适应基因。重点放在专性海洋放线菌属Salinispora和从其他海洋细菌获得的海洋适应基因的鉴定上。利用功能注释、比较基因组学和与来自高渗透环境的细菌共同进化历史的证据，确定了50多个海洋适应基因库。放线菌物种树被用来推断基因获得或丢失的可能性，以说明每个基因的分布。获得性海洋适应基因与电子传递、钠和ABC转运体以及通道和毛孔相关。此外，一个机械敏感通道基因的缺失似乎是盐霉菌株在转移到低渗透强度的培养基后不能生长的主要原因。可获得基因组序列的海洋放线菌广泛分布于放线菌系统发育树中，并与非海洋形式密切相关，这表明它们是最近独立引入海洋环境的。看来，对Salinispora spp.转运蛋白的收购。代表了一种主要的海洋适应，而基因丢失被认为是该属无法在海洋环境外生存的原因之一。这项研究揭示了革兰氏阳性细菌和革兰氏阴性细菌海洋适应的根本差异，所分析的放线菌之间没有共同的海洋适应遗传基础。
Actinobacteria represent a consistent component of most marine bacterial communities yet little is known about the mechanisms by which these Gram-positive bacteria adapt to life in the marine environment. Here we employed a phylogenomic approach to identify marine adaptation genes in marine Actinobacteria. The focus was on the obligate marine actinomycete genus Salinispora and the identification of marine adaptation genes that have been acquired from other marine bacteria. Functional annotation, comparative genomics, and evidence of a shared evolutionary history with bacteria from hyperosmotic environments were used to identify a pool of more than 50 marine adaptation genes. An Actinobacterial species tree was used to infer the likelihood of gene gain or loss in accounting for the distribution of each gene. Acquired marine adaptation genes were associated with electron transport, sodium and ABC transporters, and channels and pores. In addition, the loss of a mechanosensitive channel gene appears to have played a major role in the inability of Salinispora strains to grow following transfer to low osmotic strength media. The marine Actinobacteria for which genome sequences are available are broadly distributed throughout the Actinobacterial phylogenetic tree and closely related to non-marine forms suggesting they have been independently introduced relatively recently into the marine environment. It appears that the acquisition of transporters in Salinispora spp. represents a major marine adaptation while gene loss is proposed to play a role in the inability of this genus to survive outside of the marine environment. This study reveals fundamental differences between marine adaptations in Gram-positive and Gram-negative bacteria and no common genetic basis for marine adaptation among the Actinobacteria analyzed.
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影响因子：
14.9
作者：
Ren Q;Chen K;Paulsen IT
通讯作者：
Paulsen IT
DOI：
10.1007/s10482-008-9239-x
发表时间：
2008-06-01
影响因子：
2.6
作者：
Jensen, Paul R.;Lauro, Federico M.
通讯作者：
Lauro, Federico M.
DOI：
10.1099/ijs.0.63625-0
发表时间：
2005-09-01
影响因子：
2.8
作者：
Maldonado, LA;Fenical, W;Goodfellow, M
通讯作者：
Goodfellow, M
影响因子：
4.9
作者：
Pommier, T.;Canback, B.;Hagstrom, A.
通讯作者：
Hagstrom, A.
影响因子：
3.2
作者：
DRAPEAU, GR;MATULA, TI;MACLEOD, RA
通讯作者：
MACLEOD, RA