Znf703, a novel target of Pax3 and Zic1, regulates hindbrain and neural crest development in Xenopus.
Znf703, a novel target of Pax3 and Zic1, regulates hindbrain and neural crest development in Xenopus.
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DOI：
10.1002/dvg.23082
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Saint-Jeannet JP
中科院分区：
文献类型：
--
作者：
Hong CS;Saint-Jeannet JP
转录因子Pax 3和Zic 1对确定神经板边界和促进神经嵴形成至关重要。在一个微阵列筛选设计，以确定基因调控的Pax 3和Zic 1在非洲爪蟾，我们分离Znf 703/Nlz 1的转录抑制因子成员的NET蛋白家族。在早期神经胚阶段，znf 703在背外胚层中表达，跨越神经板和神经板边界，其表达的前边界对应于预期的后脑中的菱形3和4（r3/r4）。znf 703作为Pax 3和Zic 1的真正靶点，在胚胎中受神经板边缘诱导信号的激活，其表达依赖于Pax 3和Zic 1的功能。Znf 703吗啉介导的敲除扩大了几个后脑后基因，而Znf 703过表达完全抹杀了这些节段基因的表达，这意味着Znf 703的转录抑制活性是至关重要的模式后脑。此外，snai 2和sox 10的表达严重受损后，操作Znf 703在胚胎中的表达水平，这表明Znf 703参与神经嵴的形成下游的Pax 3和Zic 1在非洲爪蟾。
The transcription factors Pax3 and Zic1 are critical to specify the neural plate border and to promote neural crest formation. In a microarray screen designed to identify genes regulated by Pax3 and Zic1 in Xenopus we isolated Znf703/Nlz1 a transcriptional repressor member of the NET protein family. At early neurula stage znf703 is expressed in the dorsal ectoderm, spanning the neural plate and neural plate border, with an anterior boundary of expression corresponding to rhombomeres 3 and 4 (r3/r4) in the prospective hindbrain. As a bonafide target of Pax3 and Zic1, znf703 is activated by neural plate border inducing signals, and its expression depends on Pax3 and Zic1 function in the embryo. Znf703 morpholino-mediated knockdown expanded several posterior hindbrain genes, while Znf703 overexpression completely obliterated the expression of these segmental genes, signifying that the transcriptional repressor activity of Znf703 is critical to pattern the hindbrain. Furthermore, snai2 and sox10 expression was severely impaired upon manipulation of Znf703 expression levels in the embryo suggesting that Znf703 participates in neural crest formation downstream of Pax3 and Zic1 in Xenopus.
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DOI：
10.1242/dev.028043
发表时间：
2009-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ji SJ;Periz G;Sockanathan S
通讯作者：
Sockanathan S
影响因子：
4.6
作者：
Bae CJ;Jeong J;Saint-Jeannet JP
通讯作者：
Saint-Jeannet JP
影响因子：
3
作者：
Hong, Chang-Soo;Devotta, Arun;Lee, Young-Hoon;Park, Byung-Yong;Saint-Jeannet, Jean-Pierre
通讯作者：
Saint-Jeannet, Jean-Pierre
影响因子：
11.1
作者：
Holland, Daniel G.;Burleigh, Angela;Git, Anna;Goldgraben, Mae A.;Perez-Mancera, Pedro A.;Chin, Suet-Feung;Hurtado, Antonio;Bruna, Alejandro;Ali, H. Raza;Greenwood, Wendy;Dunning, Mark J.;Samarajiwa, Shamith;Menon, Suraj;Rueda, Oscar M.;Lynch, Andy G.;McKinney, Steven;Ellis, Ian O.;Eaves, Connie J.;Carroll, Jason S.;Curtis, Christina;Aparicio, Samuel;Caldas, Carlos
通讯作者：
Caldas, Carlos
影响因子：
2.5
作者：
Hoyle, J;Tang, YP;Sive, H
通讯作者：
Sive, H