The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa.
The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa.
复制标题
Hahella sp.灵菌红素的杀藻机制。
DOI：
10.1038/s41598-017-08132-5
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发表时间：
2017-08-10
影响因子：
4.6
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Yang K;Chen Q;Zhang D;Zhang H;Lei X;Chen Z;Li Y;Hong Y;Ma X;Zheng W;Tian Y;Zheng T;Xu H
近年来，铜绿微囊藻水华在全球范围内发生，造成巨大的经济损失，破坏水生生态系统。因此，有必要开发有效的、生态友好的方法来控制铜绿假单胞菌水华。在此，我们报道了灵芝菌素(PG)对铜绿假单胞菌的高效杀藻活性及其杀藻机理。PG对铜绿假单胞菌有较高的杀藻活性，72 μh的半数致死量(LD50)为5.87 g/mL。结合碘化丙啶和Annexin V-荧光素染色以及光镜和电子显微镜观察，发现存在两种与真核细胞程序性死亡相似的细胞死亡方式：坏死样和凋亡样。生化和生理分析表明，PG产生活性氧(ROS)，导致膜脂过氧化，破坏膜系统，破坏光系统的功能。蛋白质组学分析表明，许多蛋白质在PG胁迫下存在差异表达，其中大多数参与了重要的代谢过程，这可能会引发坏死性或凋亡性细胞死亡。本研究揭示了PG对铜绿假单胞菌的多种毒性机制及其在控制湖泊铜绿假单胞菌水华发生中的潜力。
In recent years, Microcystis aeruginosa blooms have occurred throughout the world, causing huge economic losses and destroying aquatic ecosystems. It is necessary to develop effective and ecofriendly methods to control M. aeruginosa blooms. Here, we report a high algicidal activity of prodigiosin (PG) against M. aeruginosa as well as the algicidal mechanism. PG showed high algicidal activity against M. aeruginosa, with a 50% lethal dose (LD50) of 5.87 μg/mL in 72 h. A combination of methods, including propidium iodide and Annexin V-fluorescein staining assays and light and electron microscopy indicated the existence of two modes of cell death with features similar to those in eukaryotic programmed cell death: necrotic-like and apoptotic-like. Biochemical and physiological analyses showed that PG generates reactive oxygen species (ROS), which induce lipid peroxidation, damage the membrane system and destroy the function of the photosystem. A proteomics analysis revealed that many proteins were differentially expressed in response to PG stress and that most of these proteins were involved in important metabolic processes, which may trigger necrotic-like or apoptotic-like cell death. The present study sheds light on the multiple toxicity mechanisms of PG on M. aeruginosa and its potential for controlling the occurrence of M. aeruginosa blooms in lakes.
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