The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging.
The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging.
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DOI：
10.1016/j.pacs.2017.11.001
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发表时间：
2018-03
期刊：
影响因子：
7.9
通讯作者：
Jokerst JV
中科院分区：
文献类型：
--
作者：
Hariri A;Lemaster J;Wang J;Jeevarathinam AS;Chao DL;Jokerst JV
我们表征了一种市售的基于LED的光声成像系统。测量LED光束轮廓、空间/时间分辨率和穿透深度。以吲哚菁绿色和亚甲基蓝作为外源性造影剂，计算其检出限。在活体小鼠中使用近红外染料（DiR）标记的人间充质干细胞证明了体内实验的能力。光声成像（派）是一种结合了光激发和超声检测的非侵入性、高分辨率混合成像模式。派可使内源性发色团（黑色素、血红蛋白等）成像。和外源性造影剂。然而，大多数当前设备使用精密且复杂的OPO激光器，其调谐和稳定性特征与广泛的临床部署不一致。随着派在医学中的应用数量的增加，迫切需要使成像设备更紧凑、便携和负担得起。在这里，便携式发光二极管为基础的光声成像（PLED-PAI）的介绍和特点的系统规格，光源表征，光声空间/时间分辨率，和穿透。该系统使用两个LED阵列连接到传统的超声换能器的侧面。LED脉冲重复率可在1 K Hz、2 K Hz、3 K Hz和4 K Hz之间调谐。轴向分辨率为0.268 mm，横向分辨率为0.55 ~ 0.59 mm。该系统可检测3.2 cm深度的铅笔芯光吸收体，吲哚菁绿色（ICG）和亚甲基蓝（MB）的检测限分别为9 μM和0.78 mM。实现标记的人间充质干细胞的体内成像以确认与小动物成像的相容性。我们在这里报告的表征可能有价值的其他团体评估市售的光声成像设备。
We characterized a commercially available LED-based photoacoustic imaging system. The LED beam profile, spatial/temporal resolution, and penetration depth were measured. Indocyanine green and methylene blue were utilized as exogenous contrast agent, and their detection limits were calculated. The capability for in vivo experiments was demonstrated using human mesenchymal stem cells labeled with a near-infrared dye (DiR) in living mice. Photoacoustic imaging (PAI) is a non-invasive, high-resolution hybrid imaging modality that combines optical excitation and ultrasound detection. PAI can image endogenous chromophores (melanin, hemoglobin, etc.) and exogenous contrast agents in different medical applications. However, most current equipment uses sophisticated and complicated OPO lasers with tuning and stability features inconsistent with broad clinical deployment. As the number of applications of PAI in medicine increases, there is an urgent need to make the imaging equipment more compact, portable, and affordable. Here, portable light emitting diode – based photoacoustic imaging (PLED-PAI) was introduced and characterized in terms of system specifications, light source characterizations, photoacoustic spatial/temporal resolution, and penetration. The system uses two LED arrays attached to the sides of a conventional ultrasound transducer. The LED pulse repetition rate is tunable between 1 K Hz, 2 K Hz, 3 K Hz, and 4 K Hz. The axial resolution was 0.268 mm, and the lateral resolution was between 0.55 and 0.59 mm. The system could detect optical absorber (pencil lead) at a depth of 3.2 cm and the detection limits of indocyanine green (ICG) and methylene blue (MB) were 9 μM and 0.78 mM. In vivo imaging of labeled human mesenchymal stem cells was achieved to confirm compatibility with small animal imaging. The characterization we report here may have value to other groups evaluating commercially available photoacoustic imaging equipment.
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