Persistence of undifferentiated spermatogonia in aged Japanese Black cattle.
Persistence of undifferentiated spermatogonia in aged Japanese Black cattle.
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DOI：
10.1111/asj.13572
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Hara K
中科院分区：
文献类型：
--
作者：
Kawahara T;Kanouchi M;Naniwa Y;Koyago M;Numabe T;Mizutani K;Tanemura K;Hara K
衰老是精子发生退化的主要危险因素。然而，年龄对公牛生精干细胞及其祖细胞的影响在很大程度上是未知的。在这里，我们报告了与年龄相关的变化，未分化和分化的精原细胞在日本黑牛几乎恒定的精子输出，通过使用精原细胞标记物。与青年牛睾丸相比，老年牛睾丸中分化型精原细胞和高分化型生精细胞数量显著减少。与此相反，未分化的精原细胞的数量保持不变，其增殖活性没有显着差异之间的年轻和老年牛睾丸。虽然在老年睾丸中仅观察到小范围的严重钙化，但在非钙化睾丸区域观察到较少的支持细胞和间质纤维化。这些结果表明，即使在老年公牛几乎恒定的精子输出，睾丸生精活性下降，而未分化的精原细胞数量保持不变。因此，我们认为未分化的精原细胞可能对牛睾丸中与年龄相关的变化具有抵抗力。由于未分化的精原细胞可能含有干细胞活性，我们的研究结果突出了未分化的精原细胞作为农业资源的潜在效用，通过移植和体外精子发生在未来的动物生产中产生精子超过自然牛的寿命。
Aging is a major risk factor for spermatogenesis deterioration. However, the influence of age on spermatogenic stem cells and their progenitors in bulls is largely unknown. Here, we report age‐related changes in undifferentiated and differentiating spermatogonia in Japanese Black cattle with nearly constant sperm output, by using spermatogonial markers. The numbers of differentiating spermatogonia and more differentiated spermatogenic cells were significantly decreased in aged bovine testes compared with those in young testes. In contrast, the number of undifferentiated spermatogonia was maintained, and their proliferative activity did not differ significantly between young and aged bovine testes. Although severe calcification was only observed to a small extent in aged testes, fewer Sertoli cells and interstitial fibrosis were observed in noncalcified testicular regions. These results suggest that, even in old bulls with nearly constant sperm output, testicular spermatogenic activity declined whereas undifferentiated spermatogonia numbers were maintained. Thus, we propose that undifferentiated spermatogonia may be resistant to age‐related changes in bovine testes. Because undifferentiated spermatogonia may contain stem cell activity, our findings highlight the potential utility of undifferentiated spermatogonia as an agricultural resource to produce spermatozoa beyond the natural bovine lifetime through transplantation and in vitro spermatogenesis in future animal production.
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影响因子：
4.8
作者：
Rebourcet D;Darbey A;Monteiro A;Soffientini U;Tsai YT;Handel I;Pitetti JL;Nef S;Smith LB;O'Shaughnessy PJ
通讯作者：
O'Shaughnessy PJ
DOI：
10.1073/pnas.2010102117
发表时间：
2020-09-29
影响因子：
11.1
作者：
Ciccarelli M;Giassetti MI;Miao D;Oatley MJ;Robbins C;Lopez-Biladeau B;Waqas MS;Tibary A;Whitelaw B;Lillico S;Park CH;Park KE;Telugu B;Fan Z;Liu Y;Regouski M;Polejaeva IA;Oatley JM
通讯作者：
Oatley JM
影响因子：
23.9
作者：
Hara, Kenshiro;Nakagawa, Toshinori;Enomoto, Hideki;Suzuki, Mikiko;Yamamoto, Masayuki;Simons, Benjamin D.;Yoshida, Shosei
通讯作者：
Yoshida, Shosei
影响因子：
2
作者：
Jiang, Huan;Zhu, Wei-Jie;Gu, Yi-Qun
通讯作者：
Gu, Yi-Qun
影响因子：
--
作者：
BERNDSTON, WE;DESJARDINS, C
通讯作者：
DESJARDINS, C