Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.).
Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.).
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外源甘氨酸氮增强生菜（Lactuca sativa L.）糖基化黄酮类化合物的积累和抗氧化活性。
DOI：
10.3389/fpls.2017.02098
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发表时间：
2017
影响因子：
5.6
通讯作者：
Huang D
中科院分区：
文献类型：
--
作者：
Yang X;Cui X;Zhao L;Guo D;Feng L;Wei S;Zhao C;Huang D
甘氨酸是自然界中最简单的氨基酸，也是土壤中含量最丰富的游离氨基酸之一，被认为是有机氮研究的模式营养素。迄今为止，许多研究集中在有机氮在植物体内的吸收、代谢和分配，但很少有人研究有机氮供应下植物的营养性能。莴苣（Lactuca sativa L.）作为全世界消费最广泛的叶菜之一，是抗氧化剂和生物活性化合物如多酚、抗坏血酸和生育酚的重要来源。在这项研究中，两个生菜品种，申选1号和Lollo Rossa，水培培养基中含有4.5，9，或18 mM甘氨酸或9 mM硝酸盐（对照）4周，和水平的健康促进化合物和抗氧化活性的生菜叶提取物进行了评估。与对照莴苣相比，甘氨酸显著降低鲜重，而与4.5或18 mM甘氨酸相比，9 mM甘氨酸显著增加鲜重。与对照组相比，甘氨酸（18 mM的参选1号; 9 mM的Lollo Rossa）显著提高了大多数抗氧化剂（包括总多酚、α-生育酚）的水平和抗氧化活性，表明适当的甘氨酸供应促进了抗氧化剂的积累和活性。甘氨酸诱导大多数糖基化槲皮素衍生物和毛地黄黄酮衍生物检测和减少一些酚酸相比，硝酸盐处理。这项研究表明，外源性甘氨酸补充剂可以战略性地用于促进健康促进化合物的积累和水培生菜的抗氧化活性，这可能会改善人类的营养。
Glycine, the simplest amino acid in nature and one of the most abundant free amino acids in soil, is regarded as a model nutrient in organic nitrogen studies. To date, many studies have focused on the uptake, metabolism and distribution of organic nitrogen in plants, but few have investigated the nutritional performance of plants supplied with organic nitrogen. Lettuce (Lactuca sativa L.), one of the most widely consumed leafy vegetables worldwide, is a significant source of antioxidants and bioactive compounds such as polyphenols, ascorbic acid and tocopherols. In this study, two lettuce cultivars, Shenxuan 1 and Lollo Rossa, were hydroponically cultured in media containing 4.5, 9, or 18 mM glycine or 9 mM nitrate (control) for 4 weeks, and the levels of health-promoting compounds and antioxidant activity of the lettuce leaf extracts were evaluated. Glycine significantly reduced fresh weight compared to control lettuce, while 9 mM glycine significantly increased fresh weight compared to 4.5 or 18 mM glycine. Compared to controls, glycine (18 mM for Shenxuan 1; 9 mM for Lollo Rossa) significantly increased the levels of most antioxidants (including total polyphenols, α-tocopherol) and antioxidant activity, suggesting appropriate glycine supply promotes antioxidant accumulation and activity. Glycine induced most glycosylated quercetin derivatives and luteolin derivatives detected and decreased some phenolic acids compared to nitrate treatment. This study indicates exogenous glycine supplementation could be used strategically to promote the accumulation of health-promoting compounds and antioxidant activity of hydroponically grown lettuce, which could potentially improve human nutrition.
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影响因子：
3.3
作者：
Clifford, Michael N.;Zheng, Wang;Kuhnert, Nikolai
通讯作者：
Kuhnert, Nikolai
影响因子：
5.6
作者：
Brunetti C;Di Ferdinando M;Fini A;Pollastri S;Tattini M
通讯作者：
Tattini M
影响因子：
50.5
作者：
Ekstrom, A. M.;Serafini, M.;Bellocco, R.
通讯作者：
Bellocco, R.
影响因子：
6.1
作者：
Clifford, MN;Knight, S;Kuhnert, N
通讯作者：
Kuhnert, N
DOI：
10.1016/j.jnutbio.2013.06.003
发表时间：
2013-11
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Babu PV;Liu D;Gilbert ER
通讯作者：
Gilbert ER