Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging.
Differential transport function of lymphatic vessels in the rat tail model and the long-term effects of Indocyanine Green as assessed with near-infrared imaging.
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DOI：
10.3389/fphys.2013.00215
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发表时间：
2013
影响因子：
4
通讯作者：
Dixon JB
中科院分区：
文献类型：
--
作者：
Weiler M;Dixon JB
引言：近红外（NIR）成像已成为一种新的成像方式，用于评估淋巴功能在体内。虽然该技术提供了以前无法获得的定量数据，但在该方法的时空能力方面仍然存在问题。我们解决了三个更重要的问题，在这里使用啮齿动物的尾巴，在淋巴文献中最广泛使用的体内模型系统之一。具体而言，我们证明了（1）淋巴管对示踪剂注射的瞬态与稳态响应，（2）并行引流相同组织空间的多个集合血管的功能特征，以及（3）荧光示踪剂对淋巴功能的长期影响，以重复功能测量。方法：用NIR对大鼠尾部进行成像，并计算排出尾部的两个收集血管的功能度量。将一氧化氮供体乳膏（GTNO）应用于尾部。此外，在初始注射时以及在第1、2和4周随访时间点，使用两种不同的NIR染料吲哚菁绿色（ICG）和LI-COR IRDye 800 CW PEG进行功能成像，之后采集两个引流淋巴结。结果和讨论：在两个收集容器之间发现显著差异，使得首先显示荧光的容器（显性）与第二容器（非显性）相比产生增强的功能度量。GTNO显着降低淋巴功能的非优势血管相比，占主导地位。注射后2周，ICG在尾部仍然可见，并伴有淋巴功能的显著丧失和引流淋巴结肿大。Licor示踪剂也在2周内保持可见。然而，该染料对淋巴功能的影响显著低于ICG，并且在任何时间点淋巴结均未增大，这表明这可能是纵向淋巴成像的更合适的造影剂。
Introduction: Near-infrared (NIR) imaging has emerged as a novel imaging modality for assessing lymphatic function in vivo. While the technique has provided quantitative data previously unavailable, questions remain in regards to the spatiotemporal capabilities of the approach. We address three of the more important issues here using the rodent tail, one of the most widely utilized in vivo model systems in the lymphatic literature. Specifically we demonstrate (1) the transient vs. steady state response of lymphatics to tracer injection, (2) the functional characteristics of multiple collecting vessels draining the same tissue space in parallel, and (3) the long-term consequences of fluorescent tracers on lymphatic function to repeated functional measurements. Methods: Rat tails were imaged with NIR and metrics of function were calculated for both collecting vessels that drain the tail. A nitric oxide donor cream (GTNO) was applied to the tail. Additionally, two different NIR dyes, indocyanine green (ICG) and LI-COR IRDye 800CW PEG, were utilized for function imaging at the time of initial injection and at 1, 2, and 4 week follow-up time points after which both draining lymph nodes were harvested. Results and Discussion: Significant differences were found between the two collecting vessels such that the vessel first showing fluorescence (dominant) produced enhanced functional metrics compared to the second vessel (non-dominant). GTNO significantly reduced lymphatic function in the non-dominant vessel compared to the dominant. ICG remained visible in the tail for 2 weeks after injection and was accompanied by significant losses in lymphatic function and enlarged draining lymph nodes. The Licor tracer also remained visible for 2 weeks. However, the dye produced significantly lower effects on lymphatic function than ICG, and lymph nodes were not enlarged at any time point, suggesting that this may be a more appropriate contrast agent for longitudinal lymphatic imaging.
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DOI：
10.1016/s1011-1344(96)07453-2
发表时间：
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影响因子：
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