Graphene as a novel matrix for the analysis of small molecules by MALDI-TOF MS.
Graphene as a novel matrix for the analysis of small molecules by MALDI-TOF MS.
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DOI：
10.1021/ac101022m
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发表时间：
2010-07-15
影响因子：
7.4
通讯作者：
Wang, Yinsheng
中科院分区：
文献类型：
--
作者：
Dong, Xiaoli;Cheng, Jinsheng;Li, Jinghong;Wang, Yinsheng
石墨烯首次被用作基质，用于使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析低分子量化合物。极性化合物，包括氨基酸，多胺，抗癌药物和核苷可以成功地分析。此外，包括类固醇在内的非极性化合物可以以高分辨率和灵敏度检测。与传统的基质相比，石墨烯对非极性化合物表现出较高的解吸/电离效率。石墨烯基质用作捕获分析物的基底，并且其在激光照射时将能量转移到分析物，这允许分析物容易地解吸/电离并且消除固有基质离子的干扰。使用石墨烯作为基质避免了分析物的碎片化，并提供了良好的重现性和高耐盐性，强调了石墨烯作为基质用于复杂样品基质中实际样品的MALDI-MS分析的潜在应用。我们还证明了使用石墨烯作为固相萃取角鲨烯的吸附剂可以大大提高检测限。这项工作不仅为石墨烯的应用开辟了新的领域，而且还为代谢研究和天然产物表征等领域的低分子量化合物的高速分析提供了新技术。
Graphene was utilized for the first time as matrix for the analysis of low-molecular weight compounds using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Polar compounds including amino acids, polyamines, anticancer drugs and nucleosides could be successfully analyzed. Additionally, nonpolar compounds including steroids could be detected with high resolution and sensitivity. Compared with conventional matrix, graphene exhibited high desorption/ionization efficiency for nonpolar compounds. The graphene matrix functions as substrate to trap analytes, and it transfers energy to the analytes upon laser irradiation, which allowed for the analytes to be readily desorbed/ionized and interference of intrinsic matrix ions to be eliminated. The use of graphene as matrix avoided the fragmentation of analytes and provided good reproducibility and high salt tolerance, underscoring the potential application of graphene as matrix for MALDI-MS analysis of practical samples in complex sample matrices. We also demonstrated that the use of graphene as adsorbent for the solid-phase extraction of squalene could improve greatly the detection limit. This work not only opens a new field for applications of graphene, but also offers a new technique for high-speed analysis of low-molecular weight compounds in areas such as metabolism research and natural products characterization.
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影响因子：
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