Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly.
Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly.
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脐动脉、脐静脉和沃顿胶来源的间充质干细胞的不同血管生成潜能
DOI：
10.1155/2017/3175748
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发表时间：
2017
影响因子：
4.3
通讯作者：
Ding L
中科院分区：
文献类型：
--
作者：
Xu L;Zhou J;Liu J;Liu Y;Wang L;Jiang R;Diao Z;Yan G;Pèault B;Sun H;Ding L
来源于脐带（UC）的人间充质干细胞是同种异体细胞治疗的有利来源。在这里，我们成功地分离了来源于三个不同的人类UC室的干细胞，包括来源于脐动脉的血管周围干细胞（UCA-PSC），来源于脐静脉的血管周围干细胞（UCV-PSC），和来源于沃顿胶质的间充质干细胞（WJ-MSC）。这些细胞具有相似的表型和向脂肪细胞、成骨细胞和神经元样细胞的分化潜能。UCA-PSC和UCV-PSC的CD 146+细胞数明显高于WJ-MSCs（P < 0.05）。体外管状结构形成实验显示，三种干细胞中UCA-PSC的管状结构和分支点最多。UCA-PSC和UCV-PSC的管腔总长度显著长于WJ-MSCs（P < 0.01）。微阵列、qRT-PCR和Western印迹分析表明，UCA-PSC具有最高的Notch配体Jagged 1（JAG 1）表达，这对血管成熟至关重要。敲低Jagged 1显著损害UCA-PSC中的血管生成。总之，UCA-PSC是用于缺血性疾病临床应用的有前景的细胞群。
Human mesenchymal stem cells derived from the umbilical cord (UC) are a favorable source for allogeneic cell therapy. Here, we successfully isolated the stem cells derived from three different compartments of the human UC, including perivascular stem cells derived from umbilical arteries (UCA-PSCs), perivascular stem cells derived from umbilical vein (UCV-PSCs), and mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs). These cells had the similar phenotype and differentiation potential toward adipocytes, osteoblasts, and neuron-like cells. However, UCA-PSCs and UCV-PSCs had more CD146+ cells than WJ-MSCs (P < 0.05). Tube formation assay in vitro showed the largest number of tube-like structures and branch points in UCA-PSCs among the three stem cells. Additionally, the total tube length in UCA-PSCs and UCV-PSCs was significantly longer than in WJ-MSCs (P < 0.01). Microarray, qRT-PCR, and Western blot analysis showed that UCA-PSCs had the highest expression of the Notch ligand Jagged1 (JAG1), which is crucial for blood vessel maturation. Knockdown of Jagged1 significantly impaired the angiogenesis in UCA-PSCs. In summary, UCA-PSCs are promising cell populations for clinical use in ischemic diseases.
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影响因子：
5.6
作者：
Kim DW;Staples M;Shinozuka K;Pantcheva P;Kang SD;Borlongan CV
通讯作者：
Borlongan CV
影响因子：
5.3
作者：
Crisan M;Corselli M;Chen WC;Péault B
通讯作者：
Péault B
影响因子：
20.3
作者：
Corselli, Mirko;Chin, Chee Jia;Peault, Bruno
通讯作者：
Peault, Bruno
影响因子：
37.8
作者：
Campagnolo P;Cesselli D;Al Haj Zen A;Beltrami AP;Kränkel N;Katare R;Angelini G;Emanueli C;Madeddu P
通讯作者：
Madeddu P
影响因子：
--
作者：
Mennan C;Wright K;Bhattacharjee A;Balain B;Richardson J;Roberts S
通讯作者：
Roberts S