Perivascular cells for regenerative medicine.
Perivascular cells for regenerative medicine.
复制标题
血管周细胞再生医学。
DOI：
10.1111/j.1582-4934.2012.01617.x
复制
发表时间：
2012-12
影响因子：
5.3
通讯作者：
Péault B
中科院分区：
文献类型：
--
作者：
Crisan M;Corselli M;Chen WC;Péault B
间质茎/基质细胞（MSC）目前是与骨，肌肉，肌肉，血管和炎症性疾病有关的最佳候选治疗细胞，尽管这些细胞仍然是异质性的，尽管我们和其他人都在典型的生物学细胞中构成了典型的细胞，但它们是典型的典型细胞，并构成了典型的典型派系，并构成了典型的细胞。人体器官可以通过定义明确的细胞表面标记来纯化。分别与平滑肌细胞中的表达相关，在周围的肌肉中，肌肉肌动蛋白（αSMA）也与最大的viss体面相反ES和晚期血管周细胞在MSC的原位和培养标记中表现出对成骨，脂肪生成和软骨细胞谱系的区分，先进的细胞可以在体外的电感状况下区分到周围的细胞，而不是使用纯粹的细胞来实现较高的细胞。
Mesenchymal stem/stromal cells (MSC) are currently the best candidate therapeutic cells for regenerative medicine related to osteoarticular, muscular, vascular and inflammatory diseases, although these cells remain heterogeneous and necessitate a better biological characterization. We and others recently described that MSC originate from two types of perivascular cells, namely pericytes and adventitial cells and contain the in situ counterpart of MSC in developing and adult human organs, which can be prospectively purified using well defined cell surface markers. Pericytes encircle endothelial cells of capillaries and microvessels and express the adhesion molecule CD146 and the PDGFRβ, but lack endothelial and haematopoietic markers such as CD34, CD31, vWF (von Willebrand factor), the ligand for Ulex europaeus 1 (UEA1) and CD45 respectively. The proteoglycan NG2 is a pericyte marker exclusively associated with the arterial system. Besides its expression in smooth muscle cells, smooth muscle actin (αSMA) is also detected in subsets of pericytes. Adventitial cells surround the largest vessels and, opposite to pericytes, are not closely associated to endothelial cells. Adventitial cells express CD34 and lack αSMA and all endothelial and haematopoietic cell markers, as for pericytes. Altogether, pericytes and adventitial perivascular cells express in situ and in culture markers of MSC and display capacities to differentiate towards osteogenic, adipogenic and chondrogenic cell lineages. Importantly, adventitial cells can differentiate into pericyte-like cells under inductive conditions in vitro. Altogether, using purified perivascular cells instead of MSC may bring higher benefits to regenerative medicine, including the possibility, for the first time, to use these cells uncultured.
登录
查看更多内容
DOI：
10.1111/j.1749-6632.2009.04967.x
发表时间：
2009-01-01
期刊：
HEMATOPOIETIC STEM CELLS VII
影响因子：
--
作者：
Crisan, Mihaela;Chen, Chien-Wen;Peault, Bruno
通讯作者：
Peault, Bruno
影响因子：
7.8
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
通讯作者：
Cossu, G
影响因子：
37.8
作者：
Campagnolo P;Cesselli D;Al Haj Zen A;Beltrami AP;Kränkel N;Katare R;Angelini G;Emanueli C;Madeddu P
通讯作者：
Madeddu P
影响因子：
4.1
作者：
ASHTON, N;DEOLIVEI.F
通讯作者：
DEOLIVEI.F
影响因子：
37.8
作者：
Dar, Ayelet;Domev, Hagit;Itskovitz-Eldor, Joseph
通讯作者：
Itskovitz-Eldor, Joseph