Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation.
Reversible silencing of lumbar spinal interneurons unmasks a task-specific network for securing hindlimb alternation.
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DOI：
10.1038/s41467-017-02033-x
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发表时间：
2017-12-06
影响因子：
16.6
通讯作者：
Magnuson DSK
中科院分区：
文献类型：
--
作者：
Pocratsky AM;Burke DA;Morehouse JR;Beare JE;Riegler AS;Tsoulfas P;States GJR;Whittemore SR;Magnuson DSK
腰脊髓的神经回路控制着运动的两个主要特征：节律和模式，反映了肢体内和肢体间的运动。这些功能在功能上被组织成一个层次结构，以一种刻板的、与速度相关的方式精确控制步进。在这里，我们证明运动模式的特定组成部分可以被独立操纵。沉默投射到 L5 的脊髓 L2 中间神经元会选择性地破坏后肢交替，从而从原本完整的网络中出现步行到跳跃的连续过程。这种扰动与速度无关，并且在每一步中自发发生，不会破坏多关节运动或前肢交替，也不会转化为非负重运动活动。脚步的基本节奏以及速度和时空特征之间的通常关系仍然存在。这些数据表明，后肢交替可以独立于步进的其他核心特征进行操作，揭示了在其他精确控制系统中的惊人自由度。运动期间的肢体内和肢体间协调由分层组织的腰椎网络控制。在这里，作者表明，脊髓 L2-L5 中间神经元的可逆沉默会特异性地破坏后肢交替，从而导致步行到跳跃的连续过程。
Neural circuitry in the lumbar spinal cord governs two principal features of locomotion, rhythm and pattern, which reflect intra- and interlimb movement. These features are functionally organized into a hierarchy that precisely controls stepping in a stereotypic, speed-dependent fashion. Here, we show that a specific component of the locomotor pattern can be independently manipulated. Silencing spinal L2 interneurons that project to L5 selectively disrupts hindlimb alternation allowing a continuum of walking to hopping to emerge from the otherwise intact network. This perturbation, which is independent of speed and occurs spontaneously with each step, does not disrupt multi-joint movements or forelimb alternation, nor does it translate to a non-weight-bearing locomotor activity. Both the underlying rhythm and the usual relationship between speed and spatiotemporal characteristics of stepping persist. These data illustrate that hindlimb alternation can be manipulated independently from other core features of stepping, revealing a striking freedom in an otherwise precisely controlled system. Intra- and interlimb coordination during locomotion is governed by hierarchically organized lumbar spinal networks. Here, the authors show that reversible silencing of spinal L2–L5 interneurons specifically disrupts hindlimb alternation leading to a continuum of walking to hopping.
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DOI：
10.1073/pnas.1419045111
发表时间：
2014-11-25
影响因子：
11.1
作者：
Akay, Turgay;Tourtellotte, Warren G.;Jessell, Thomas M.
通讯作者：
Jessell, Thomas M.
影响因子：
2.5
作者：
Cowley, KC;Schmidt, BJ
通讯作者：
Schmidt, BJ
影响因子：
5.3
作者：
Dougherty, Kimberly J.;Kiehn, Ole
通讯作者：
Kiehn, Ole
影响因子：
16.2
作者：
Fremeau, RT;Troyer, MD;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
--
作者：
Butt, SJB;Lebret, JM;Kiehn, O
通讯作者：
Kiehn, O