In search of the Golden Fleece: unraveling principles of morphogenesis by studying the integrative biology of skin appendages.
In search of the Golden Fleece: unraveling principles of morphogenesis by studying the integrative biology of skin appendages.
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DOI：
10.1039/c0ib00108b
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Chuong CM
中科院分区：
文献类型：
--
作者：
Hughes MW;Wu P;Jiang TX;Lin SJ;Dong CY;Li A;Hsieh FJ;Widelitz RB;Chuong CM
金羊毛的神话故事象征着皮肤附属物神奇的再生能力。与多才多艺的阿尔戈英雄对金羊毛的冒险追求类似，今天我们也需要一种综合的多学科方法来了解皮肤附属物发育，再生和进化过程中的细胞和分子过程。为此，我们已经探索了几个方面的皮肤附属物生物学，有助于图灵激活剂/抑制剂模型在羽毛图案的形成，在器官形状的决定，宏观环境调节的干细胞再生的头发波，和潜在的新的分子途径在羽毛的形态进化的干细胞的拓扑生物学安排。在这里，我们展示了我们目前的综合生物学努力，以解开复杂的细胞行为模式的干细胞和控制区域特异性的皮肤附件。我们使用羽毛/鳞片组织重组来展示感受态和诱导的时间控制。来自不同身体区域的羽毛用于研究皮肤区域特异性。转录组微阵列的生物信息学分析显示了候选分子通路的潜在参与。我们进一步表明Hox基因表现出一些区域特异性表达模式。为了使真实的时间事件可视化，我们应用延时电影、共聚焦显微镜和多光子显微镜来分析培养的鸡胚皮肤外植体的形态发生。这些现代成像技术在三维中意外地揭示了细胞外基质分子的复杂细胞流动和组织。虽然这些方法还处于初步阶段，但这一观点强调了我们面临的挑战和我们将使用的新的综合工具。未来的工作将遵循这些线索，发展系统生物学的观点和理解的形态发生的原则，管理外胚层器官的发展和再生。
The mythological story of the Golden Fleece symbolizes the magical regenerative power of skin appendages. Similar to the adventurous pursuit of the Golden Fleece by the multi-talented Argonauts, today we also need an integrated multi-disciplined approach to understand the cellular and molecular processes during development, regeneration and evolution of skin appendages. To this end, we have explored several aspects of skin appendage biology that contribute to the Turing activator / inhibitor model in feather pattern formation, the topo-biological arrangement of stem cells in organ shape determination, the macro-environmental regulation of stem cells in regenerative hair waves, and potential novel molecular pathways in the morphological evolution of feathers. Here we show our current integrative biology efforts to unravel the complex cellular behavior in patterning stem cells and the control of regional specificity in skin appendages. We use feather / scale tissue recombination to demonstrate the timing control of competence and inducibility. Feathers from different body regions are used to study skin regional specificity. Bioinformatic analyses of transcriptome microarrays show the potential involvement of candidate molecular pathways. We further show Hox genes exhibit some region specific expression patterns. To visualize real time events, we applied time-lapse movies, confocal microscopy and multiphoton microscopy to analyze the morphogenesis of cultured embryonic chicken skin explants. These modern imaging technologies reveal unexpectedly complex cellular flow and organization of extracellular matrix molecules in three dimensions. While these approaches are in preliminary stages, this perspective highlights the challenges we face and new integrative tools we will use. Future work will follow these leads to develop a systems biology view and understanding in the morphogenetic principles that govern the development and regeneration of ectodermal organs.
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影响因子：
82.9
作者：
Brown, EB;Campbell, RB;Jain, RK
通讯作者：
Jain, RK
影响因子：
3
作者：
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通讯作者：
Mueller-Roeber B
影响因子：
3.6
作者：
Al-Nuaimi Y;Baier G;Watson RE;Chuong CM;Paus R
通讯作者：
Paus R
影响因子：
6.5
作者：
Chang, CH;Yu, MK;Chuong, CM
通讯作者：
Chuong, CM
影响因子：
2.7
作者：
Crowe, R;Niswander, L
通讯作者：
Niswander, L