Factorial analyses of photopolymerizable thermoresponsive composite hydrogels for protein delivery.
Factorial analyses of photopolymerizable thermoresponsive composite hydrogels for protein delivery.
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DOI：
10.1016/j.nano.2008.11.003
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发表时间：
2009-09
影响因子：
5.4
通讯作者：
Nguyen, Kytai T.
中科院分区：
文献类型：
--
作者：
Sabnis, Abhimanyu;Wadajkar, Aniket S.;Aswath, Pranesh;Nguyen, Kytai T.
一种智能蛋白质递送系统的伤口愈合应用开发使用复合纳米粒子水凝胶，可以释放蛋白质在温度响应的方式。该体系也可以在紫外光和Irgacure 2959光引发剂存在下原位形成。该系统由温度敏感的聚（N-异丙基丙烯酰胺-共-丙烯酰胺）（PNIPAM-AAm）纳米粒子嵌入在聚（乙二醇）二丙烯酸酯（PEGDA）矩阵。进行因子分析以评估PEGDA浓度（10%和15%w/v）和PEGDA分子量（3.4kDa和8 kDa）以及PNIPAM-AAm纳米颗粒浓度（2%和4%w/v）和温度（23°C和40°C）对水凝胶的蛋白质释放曲线和溶胀比的影响。结果表明，PNIPAM-AAm纳米粒子浓度和温度是影响蛋白质释放的最重要因素。PEGDA分子量是影响蛋白质释放的最重要因素。这也是控制水凝胶溶胀比的重要因素。进一步开发双层水凝胶以产生具有更好的缓释的蛋白质递送系统。这些发现提高了我们对复合水凝胶系统的理解，并将有助于定制具有所需释放曲线的未来系统。
A smart protein delivery system for wound healing applications was developed using composite nanoparticle hydrogels that can release protein in a temperature-responsive manner. This system can also be formed in situ in the presence of ultraviolet light and Irgacure 2959 photoinitiator. The system consists of temperature sensitive poly(N-isopropylacrylamide-co-acrylamide) (PNIPAM-AAm) nanoparticles embedded in a poly(ethylene glycol) diacrylate (PEGDA) matrix. A factorial analysis was performed to evaluate the effects of PEGDA concentration (10% and 15% w/v) and PEGDA molecular weight (3.4 kDa and 8 kDa), as well as PNIPAM-AAm nanoparticle concentration (2% and 4% w/v) and temperature (23°C and 40°C) on the protein release profiles and swelling ratios of the hydrogels. Results indicate PNIPAM-AAm nanoparticle concentration and temperature were the most important factors affecting the protein release during the burst release phase. Additionally, PEGDA molecular weight was the most important factor affecting the protein release in the plateau region. It was also an important factor that controlled the hydrogel swelling ratio. A dual layered hydrogel was further developed to produce a protein delivery system with a better sustained release. These findings have improved our understanding of the composite hydrogel systems and will help in tailoring future systems with desired release profiles.
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影响因子：
--
作者：
Gonzalez, AL;Gobin, AS;Smith, CW
通讯作者：
Smith, CW
影响因子：
14
作者：
Ishihara, M;Nakanishi, K;Kurita, A
通讯作者：
Kurita, A
影响因子：
6
作者：
Caykara, T;Kiper, S;Demirel, G
通讯作者：
Demirel, G
影响因子：
3.3
作者：
Cruise, GM;Hegre, OD;Hubbell, JA
通讯作者：
Hubbell, JA
影响因子：
3.7
作者：
BAE, YH;OKANO, T;KIM, SW
通讯作者：
KIM, SW