Enamel matrix derivative expedites osteogenic differentiation of BMSCs via Wnt/β-catenin pathway in high glucose microenvironment
Enamel matrix derivative expedites osteogenic differentiation of BMSCs via Wnt/β-catenin pathway in high glucose microenvironment
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牙釉质基质衍生物在高葡萄糖微环境中通过 Wnt/β-连环蛋白途径加速 BMSC 的成骨分化
DOI：
10.1007/s00774-022-01318-6
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发表时间：
2022-03
影响因子：
3.3
通讯作者：
Qiang Dong
中科院分区：
文献类型：
--
作者：
MaoHua Meng;Qian Xia;Ying Li;Xin Chen;QinYing Wang;JingQiao Chen;XingXing Xu;Huan Wang;JiaYu Shu;Jing Lu;Lu Cheng;ZhaoYang Ye;Bin Song;Qiang Dong
目的：探讨釉基质衍生物（elaminmatrixderivatives，EMD）对高糖微环境下骨髓间充质干细胞（bonemarrowmesenchymalstemcells，BMSCs）增殖和成骨分化的影响，并通过Wnt/β-catenin信号通路的相互作用，探讨EMD对高糖微环境下BMSCs增殖和成骨分化的影响。用不同浓度的EMD在HG中处理细胞，以确定其增殖和成骨分化的最佳浓度。观察细胞生长曲线和细胞周期变化，检测Wnt/β-catenin通路中Osterix、Runx 2、COL-I、早期成骨指标、钙盐沉积和β-catenin蛋白的表达。在诱导成骨的细胞中加入Wnt/β-catenin通路抑制剂（XAV-939）后，检测β-catenin与Osterix的结合情况。不同浓度的EMD均能促进HG中细胞的增殖和成骨诱导，以75μg/mL的EMD效果最好。高糖可促进骨髓基质细胞增殖，并使流式细胞仪碘化丙啶指数升高，这一作用通过EMD得以加强。骨髓基质干细胞诱导成骨后，Osterix、Runx 2、CoL-1、早期成骨指数和钙盐沉积均降低，但通过EMD诱导成骨后，Osterix、Runx 2、CoL-1和钙盐沉积均升高。β-Catenin在高糖组最低，EMD后升高。加入XAV-939后，β-连环蛋白和下游（Osterix和Runx 2）减少。结论EMD可能通过激活HG中Wnt/β-catenin信号通路促进BMSCs向成骨细胞分化。
Introduction :The infuence of enamel matrix derivative (EMD) on proliferation and osteogenic diferentiation of bone marrow mesenchymal stem cells (BMSCs) was explored in high glucose (HG) microenvironment with interaction of Wnt/β-catenin pathway.materials and Methods: Extraction of BMSCs from Sprague–Dawley rats, culture, and identifcation were manifested. The cells were treated with diferent concentration of EMD in HG to fgure out the most available concentration for proliferation and osteogenic diferentiation. Then, observation of cell growth curve and cell cycle changes, and detection of Osterix, runt-related transcription factor 2 (Runx2), COL-I, early osteogenic indexes, Calcium salt deposition, and β-catenin protein in Wnt/β-catenin pathway were assured. After adding Wnt/β-catenin pathway inhibitor (XAV-939) in the cells with osteogenesis induction, detection of binding of β-catenin to Osterix was clarifed.Results :Via identifcation BMSCs cultured in vitro was qualifed. Diferent concentrations of EMD could accelerate cell proliferation in HG and osteogenesis induction, and 75μg/mL EMD had the best efect. The HG augmented BMSCs proliferation and the propidium iodide index of fow cytometry cycle was elevated in HG, which were strengthened via the EMD. After BMSCs’ osteogenesis induction, Osterix, Runx2, CoL-1, early osteogenic indexes, and calcium salt deposition were reduced, but elevated via EMD. β-Catenin was the lowest in the HG, but elevated after EMD. After addition of XAV-939, reduction of β-catenin and the downstream (Osterix and Runx2) were manifested. Detection of binding protein bands was in β-catenin and Osterix of the HG after EMD treatment.Conclusion EMD may facilitate the osteogenic diferentiation of BMSCs via activating the Wnt/β-catenin pathway in HG..
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影响因子：
5.1
作者：
Guariguata, L.;Whiting, D. R.;Shaw, J. E.
通讯作者：
Shaw, J. E.
影响因子：
3.7
作者：
Takeda K;Mizutani K;Matsuura T;Kido D;Mikami R;Noda M;Buranasin P;Sasaki Y;Izumi Y
通讯作者：
Izumi Y
影响因子：
4
作者：
Komori, T
通讯作者：
Komori, T
影响因子：
4.3
作者：
Correa, Monica Grazieli;Gomes Campos, Mirella L.;Sallum, Enilson A.
通讯作者：
Sallum, Enilson A.
影响因子：
12.4
作者：
Zhao, ZR;Zhao, M;Franceschi, RT
通讯作者：
Franceschi, RT