A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry.
A Phox2b BAC Transgenic Rat Line Useful for Understanding Respiratory Rhythm Generator Neural Circuitry.
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DOI：
10.1371/journal.pone.0132475
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Onimaru H
中科院分区：
文献类型：
--
作者：
Ikeda K;Takahashi M;Sato S;Igarashi H;Ishizuka T;Yawo H;Arata S;Southard-Smith EM;Kawakami K;Onimaru H
呼吸神经中枢的关键作用是产生呼吸节律，通过控制动脉血pCO 2/pH和pO 2水平维持体内平衡。负责新生大鼠呼吸节律产生的神经元网络位于延髓腹侧，由两组组成：面旁呼吸组（pFRG）和前Bötinger复合体组（preBötC）。pFRG在后斜方核（RTN）中部分重叠，这最初在成年猫和大鼠中发现。RTN/pFRG中的部分吸气前（Pre-I）神经元作为中枢化学感受器神经元，CO2敏感的Pre-I神经元表达同源框基因Phox 2b。Phox 2b编码一种转录因子，对感觉运动内脏回路的发育至关重要。人PHOX 2B的突变导致先天性低通气综合征，其特征在于对高碳酸血症的缓解反应迟钝。在这里，我们描述了一种新的转基因（Tg）大鼠窝藏荧光标记的前-I神经元的RTN/pFRG的一代。此外，Tg大鼠在自主肠神经元和颈动脉体中显示荧光信号。由于Tg大鼠在发育过程中在PHOX 2B阳性细胞中表达诱导型Cre重组酶，因此它是一种潜在的强大工具，可用于解剖发育过程中呼吸神经网络的全貌，并用于识别成人中枢和外周神经系统中的CO2/O2传感器分子。
The key role of the respiratory neural center is respiratory rhythm generation to maintain homeostasis through the control of arterial blood pCO2/pH and pO2 levels. The neuronal network responsible for respiratory rhythm generation in neonatal rat resides in the ventral side of the medulla and is composed of two groups; the parafacial respiratory group (pFRG) and the pre-Bötzinger complex group (preBötC). The pFRG partially overlaps in the retrotrapezoid nucleus (RTN), which was originally identified in adult cats and rats. Part of the pre-inspiratory (Pre-I) neurons in the RTN/pFRG serves as central chemoreceptor neurons and the CO2 sensitive Pre-I neurons express homeobox gene Phox2b. Phox2b encodes a transcription factor and is essential for the development of the sensory-motor visceral circuits. Mutations in human PHOX2B cause congenital hypoventilation syndrome, which is characterized by blunted ventilatory response to hypercapnia. Here we describe the generation of a novel transgenic (Tg) rat harboring fluorescently labeled Pre-I neurons in the RTN/pFRG. In addition, the Tg rat showed fluorescent signals in autonomic enteric neurons and carotid bodies. Because the Tg rat expresses inducible Cre recombinase in PHOX2B-positive cells during development, it is a potentially powerful tool for dissecting the entire picture of the respiratory neural network during development and for identifying the CO2/O2 sensor molecules in the adult central and peripheral nervous systems.
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DOI：
10.1126/science.1190721
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gourine AV;Kasymov V;Marina N;Tang F;Figueiredo MF;Lane S;Teschemacher AG;Spyer KM;Deisseroth K;Kasparov S
通讯作者：
Kasparov S
DOI：
10.1007/s00424-014-1633-9
发表时间：
2015-05
期刊：
Pflugers Archiv : European journal of physiology
影响因子：
--
作者：
Bayliss DA;Barhanin J;Gestreau C;Guyenet PG
通讯作者：
Guyenet PG
DOI：
10.1006/bbrc.1997.7124
发表时间：
1997-08-28
影响因子：
3.1
作者：
Feil, R;Wagner, J;Chambon, P
通讯作者：
Chambon, P
影响因子：
56.9
作者：
Gray, PA;Rekling, JC;Feldman, JL
通讯作者：
Feldman, JL
DOI：
10.1098/rstb.2009.0085
发表时间：
2009-09-12
影响因子：
6.3
作者：
Dubreuil, Veronique;Barhanin, Jacques;Brunet, Jean-Francois
通讯作者：
Brunet, Jean-Francois