Reducing expression of GluN1(0XX) subunit splice variants of the NMDA receptor interferes with spatial reference memory.
Reducing expression of GluN1(0XX) subunit splice variants of the NMDA receptor interferes with spatial reference memory.
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DOI：
10.1016/j.bbr.2012.02.014
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发表时间：
2012-05-01
影响因子：
2.7
通讯作者：
Magnusson, Kathy R.
中科院分区：
文献类型：
--
作者：
Das, Siba R.;Jensen, Ross;Kelsay, Rian;Shumaker, Michelle;Bochart, Rachele;Brim, Brenna;Zamzow, Daniel;Magnusson, Kathy R.
NMDA受体的GluN 1亚基在其表达模式中显示出与年龄相关的变化，其中一些与小鼠的空间记忆表现相关。老年C57 BL/6小鼠显示出与年龄相关的GluN 1亚基剪接变体（缺乏N末端剪接盒GluN 10 XX（GluN 1-a））mRNA表达的增加。这种表达的增加与参考和工作记忆任务的良好表现有关。本研究进行，以确定是否GluN 10 XX剪接变异体所需的良好性能在年轻小鼠的参考记忆任务。将对GluN 10 XX剪接变体特异性的siRNA、对照siRNA或单独的载体双侧注射到小鼠的腹外侧眶皮质中。第四组小鼠没有接受任何注射。从注射后5天开始，在Morris水迷宫中测试小鼠在4天内在空间参考记忆、联想记忆和认知灵活性任务中的表现。在GluN 10 XX siRNA处理后，小鼠腹外侧眶皮质内GluN 10 XX剪接变体的mRNA表达降低10 - 19%。与注射对照siRNA、载体和/或未治疗的小鼠相比，在接受针对GluN 10 XX剪接变体的siRNA的小鼠中观察到参考记忆测试的前半部分内的表现下降。这些结果表明，GluN 10 XX剪接变异体在眼眶区域的早期收购和/或巩固空间参考记忆的作用。
The GluN1 subunit of the NMDA receptor shows age-related changes in its expression pattern, some of which correlate with spatial memory performance in mice. Aged C57BL/6 mice show an age-related increase in mRNA expression of GluN1 subunit splice variants that lack the N terminal splice cassette, GluN10XX (GluN1-a). This increase in expression is associated with good performance in reference and working memory tasks. The present study was undertaken to determine if GluN10XX splice variants are required for good performance in reference memory tasks in young mice. Mice were bilaterally injected with either siRNA specific for GluN10XX splice variants, control siRNA or vehicle alone into ventro-lateral orbital cortices. A fourth group of mice did not receive any injections. Starting five days post-injection, mice were tested for their performance in spatial reference memory, associative memory and cognitive flexibility tasks over 4 days in the Morris water maze. There was a 10 –19% reduction in mRNA expression for GluN10XX splice variants within the ventro-lateral orbital cortices in mice following GluN10XX siRNA treatment. Declines in performance within the first half of reference memory testing were seen in the mice receiving siRNA against the GluN10XX splice variants, as compared to the mice injected with control siRNA, vehicle and/or no treatment. These results suggest a role for the GluN10XX splice variants in orbital regions for early acquisition and/or consolidation of spatial reference memory.
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影响因子：
1.9
作者：
GALLAGHER, M;BURWELL, RD;BURCHINAL, M
通讯作者：
BURCHINAL, M
影响因子：
2.9
作者：
Das, Siba R.;Magnusson, Kathy R.
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Magnusson, Kathy R.
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作者：
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