Pistillody mutant reveals key insights into stamen and pistil development in wheat (Triticum aestivum L.).
Pistillody mutant reveals key insights into stamen and pistil development in wheat (Triticum aestivum L.).
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DOI：
10.1186/s12864-015-1453-0
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发表时间：
2015-03-19
期刊：
影响因子：
4.4
通讯作者：
Jang Z
中科院分区：
文献类型：
--
作者：
Yang Z;Peng Z;Wei S;Liao M;Yu Y;Jang Z
雌蕊突变体小麦（Triticum aestivum L.）植物 HTS-1 表现出雄蕊向雌蕊或雌蕊样结构的同源转化。与普通小麦品种不同，HTS-1 每朵花可产生 3 至 6 个雌蕊，有可能提高产量。因此，HTS-1在小麦花发育的研究中具有很高的价值。在本研究中，我们对 HTS-1 植物的雌蕊 (PS) 和雌蕊 (P) 以及非雌蕊对照品种中国春 TP 的雄蕊 (S) 的转录组进行了 RNA 测序，以深入了解小麦雌蕊和雄蕊的发育。从 PS、P 和 S 中获得了大约 40 Gb 的处理读数。从头组装产生了 121,210 个假定的 unigene，平均长度为 695 bp。在这些高质量的unigenes中，有59,199个（48.84％）与现有基因模型至少有一个显着匹配。 PS vs. P、PS vs. S、P vs. S 中分别鉴定出 23、263 和 553 个差异表达基因，表达差异大于 5 倍。差异表达基因中，有206个与雄蕊和雌蕊发育高度相关。这些基因包括 WM27B、DL、YAB1、YABBY4、WM 5、CER 1 和 WBLH1，它们与花发育有关。通过实时定量逆转录PCR确认了25个差异表达基因的表达模式。通过对该转录组资源的分析，我们能够表征基因表达谱，检查差异基因表达，并生成与小麦雄蕊和雌蕊发育相关的候选基因列表。这项工作对于小麦基因组资源的开发具有重要意义，并为小麦雄蕊和雌蕊发育的分子机制提供了重要的见解。本文的在线版本 (doi:10.1186/s12864-015-1453-0) 包含补充材料，可供授权用户使用。
The pistillody mutant wheat (Triticum aestivum L.) plant HTS-1 exhibits homeotic transformation of stamens into pistils or pistil-like structures. Unlike common wheat varieties, HTS-1 produces three to six pistils per floret, potentially increasing the yield. Thus, HTS-1 is highly valuable in the study of floral development in wheat. In this study, we conducted RNA sequencing of the transcriptomes of the pistillody stamen (PS) and the pistil (P) from HTS-1 plants, and the stamen (S) from the non-pistillody control variety Chinese Spring TP to gain insights into pistil and stamen development in wheat. Approximately 40 Gb of processed reads were obtained from PS, P, and S. De novo assembly yielded 121,210 putative unigenes, with a mean length of 695 bp. Among these high-quality unigenes, 59,199 (48.84%) had at least one significant match with an existing gene model. A total of 23, 263, and 553 differentially expressed genes were identified in PS vs. P, PS vs. S, and P vs. S, respectively, with differences in expression greater than five-fold. Among the differentially expressed genes, 206 were highly correlated with stamen and pistil development. These genes include WM27B, DL, YAB1, YABBY4, WM 5, CER 1, and WBLH1, which have been implicated in flower development. The expression patterns of 25 differentially expressed genes were confirmed through quantitative real-time reverse transcription PCR. Analysis of this transcriptome resource enabled us to characterize gene expression profiles, examine differential gene expression, and produce a candidate gene list related to wheat stamen and pistil development. This work is significant for the development of genomic resources for wheat, and provides important insights into the molecular mechanisms of wheat stamen and pistil development. The online version of this article (doi:10.1186/s12864-015-1453-0) contains supplementary material, which is available to authorized users.
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影响因子：
5.1
作者：
Jang, S;Hur, J;An, G
通讯作者：
An, G
影响因子：
3.1
作者：
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
2
作者：
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通讯作者：
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影响因子：
1.9
作者：
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通讯作者：
TSUNEWAKI, K