Hemocompatibility of polymeric nanostructured surfaces.
Hemocompatibility of polymeric nanostructured surfaces.
复制标题
聚合物纳米结构表面的血流相容性。
DOI：
10.1080/09205063.2013.777228
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Popat KC
中科院分区：
文献类型：
--
作者：
Leszczak V;Smith BS;Popat KC
组织整合是诱导移植耐受的重要特性，然而，生物材料表面的血液相容性在植入物的最终成功中也起着重要作用。因此，为了诱导移植耐受，了解血液成分与材料表面的相互作用至关重要。在这项研究中，我们研究了关键的血清蛋白的吸附，在体外粘附和激活血小板和凝血动力学的全血上的平聚己内酯（PCL）表面，纳米线（NW）表面和纳米线（NF）表面。以前的研究表明，聚合物纳米结构表面改善细胞粘附，增殖和活力;然而，目前还不清楚这些聚合物纳米结构表面如何与血液及其成分相互作用。蛋白质吸附结果表明，虽然PCL，NW和NF表面上的总白蛋白吸附没有显着差异，NW表面具有较高的总纤维蛋白原和免疫球蛋白-G吸附相比，NF和PCL表面。相比之下，与PCL和NW表面相比，NF表面具有更高的表面FIB和IgG吸附。血小板粘附和活力研究显示，与PCL和NW表面相比，NF表面上的血小板粘附和聚集更多。血小板活化研究表明，NW表面具有最高百分比的未活化血小板，而NF表面具有最高百分比的完全活化血小板。全血凝血结果表明，与PCL和NF表面相比，NW表面在凝血过程中保持增加的游离血红蛋白量，表明其表面上的凝血较少且凝血速率较慢。
Tissue integration is an important property when inducing transplant tolerance, however, the hemocompatibility of the biomaterial surface also plays an important role in the ultimate success of the implant. Therefore, in order to induce transplant tolerance, it is critical to understand the interaction of blood components with the material surfaces. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets and clotting kinetics of whole blood on flat polycaprolactone (PCL) surfaces, nanowire (NW) surfaces and nanofiber (NF) surfaces. Previous studies have shown that polymeric nanostructured surfaces improve cell adhesion, proliferation and viability; however it is unclear how these polymeric nanostructured surfaces interact with the blood and its components. Protein adsorption results indicate that while there were no significant differences in total albumin adsorption on PCL, NW and NF surfaces, NW surfaces had higher total fibrinogen and immunoglobulin-G adsorption compared to NF and PCL surfaces. In contrast, NF surfaces had higher surface FIB and IgG adsorption compared to PCL and NW surfaces. Platelet adhesion and viability studies show more adhesion and clustering of platelets on the NF surfaces as compared to PCL and NW surfaces. Platelet activation studies reveal that NW surfaces have the highest percentage of unactivated platelets, whereas NF surfaces have the highest percentage of fully activated platelets. Whole blood clotting results indicate that NW surfaces maintain an increased amount of free hemoglobin during the clotting process compared to PCL and NF surface, indicating less clotting and slower rate of clotting on their surfaces.
登录
查看更多内容
软骨组织工程用纳米纤维 PCL-b-PLLA 支架的微观结构和性能
影响因子：
3.1
作者：
He, Liumin;Liu, Bin;Ramakrishna, S.
通讯作者：
Ramakrishna, S.
影响因子：
9.7
作者：
Andukuri, Adinarayana;Kushwaha, Meenakshi;Tambralli, Ajay;Anderson, Joel M.;Dean, Derrick R.;Berry, Joel L.;Sohn, Young Doug;Yoon, Young-Sup;Brott, Brigitta C.;Jun, Ho-Wook
通讯作者：
Jun, Ho-Wook
影响因子：
9.7
作者：
Bechara, Samuel;Wadman, Lucas;Popat, Ketul C.
通讯作者：
Popat, Ketul C.
影响因子：
11.2
作者：
Cooper, Ashleigh;Bhattarai, Narayan;Zhang, Miqin
通讯作者：
Zhang, Miqin
影响因子：
9.7
作者：
Li, Wan-Ju;Cooper, James A., Jr.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.