Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation.
Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation.
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DOI：
10.1016/j.actbio.2011.04.009
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发表时间：
2011-07
影响因子：
9.7
通讯作者：
Popat, Ketul C.
中科院分区：
文献类型：
--
作者：
Bechara, Samuel;Wadman, Lucas;Popat, Ketul C.
干细胞仍然是治疗各种神经系统紊乱和疾病的最令人兴奋和有利可图的选择之一。虽然在成人中存在神经干细胞，但外周和中枢神经系统自我修复的能力充其量是有限的。因此，迫切需要组织工程方法来解决神经系统紊乱和疾病。在这项研究中，我们开发了具有高纵横比纳米线控制阵列的导电表面，用于神经干细胞的生长和维持。纳米线表面由聚己内酯制成，采用新型纳米模板技术，并涂有导电聚合物聚吡咯。利用扫描电镜和x射线光电子能谱对聚吡咯纳米线表面进行了表征。此外，还测量了聚吡咯涂层纳米线的表面电阻。以C17.2神经干细胞为研究对象，观察聚吡咯包被纳米线表面对细胞粘附、增殖和分化的促进作用。本研究结果表明，与对照表面相比，聚吡咯涂层纳米线表面的细胞粘附和增殖明显更高。多吡咯纳米线表面的分化潜力也通过免疫染色来评估，当NSCs分化成各自的神经谱系时，关键神经元标记物表达。
Stem cells still remain one of the most exciting and lucrative options for treatment of variety of nervous system disorders and diseases. Although there are neural stem cells present in adults, the ability of both the peripheral and central nervous system for self-repair is limited at best. As such, there is a great need for a tissue engineering approach to solve nervous system disorders and diseases. In this study, we have developed electrically conductive surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of neural stem cells. The nanowire surfaces were fabricated from polycaprolactone using a novelnanotemplating technique, and were coated with an electrically conductive polymer, polypyrrole. The polypyrrole coated nanowire surfaces were characterized using scanning electron microscopy and X-ray photoelectron spectroscopy. Additionally, the surface resistance of polypyrrole coated nanowire surfaces was measured. C17.2 neural stem cells were used to evaluate the efficacy of the polypyrrole coated nanowire surfaces to promote cell adhesion, proliferation, and differentiation. The results presented here indicate significantly higher cellular adhesion and proliferation on polypyrrole coated nanowire surfaces as compared to control surfaces. The differentiation potential of polypyrrole nanowire surfaces was also evaluated by immunostaining key neuronal markers that are expressed when NSCs differentiate into their respective neural lineages.
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影响因子：
5.3
作者：
Becker, Daniel;Gary, Devin S.;Rosenzweig, Ephron S.;Grill, Warren M.;McDonald, John W.
通讯作者：
McDonald, John W.
影响因子：
9.7
作者：
Gupta, Deepika;Venugopal, J.;Ramakrishna, S.
通讯作者：
Ramakrishna, S.
DOI：
10.1016/j.nurt.2010.07.006
发表时间：
2010-10
期刊：
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Kimelberg HK;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
19
作者：
Gomez, Natalia;Lee, Jae Y.;Schmidt, Christine E.
通讯作者：
Schmidt, Christine E.
影响因子：
9
作者：
Fozdar DY;Lee JY;Schmidt CE;Chen S
通讯作者：
Chen S