Intramuscular Exposure of Macaca fascicularis to Low Doses of Low Passage- or Cell Culture-Adapted Sudan Virus or Ebola Virus.
Intramuscular Exposure of Macaca fascicularis to Low Doses of Low Passage- or Cell Culture-Adapted Sudan Virus or Ebola Virus.
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巨麦卡筋膜内肌内暴露于低剂量的低通过或细胞培养的苏丹病毒或埃博拉病毒。
DOI：
10.3390/v10110642
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发表时间：
2018-11-16
期刊：
影响因子：
--
通讯作者：
Griffiths A
中科院分区：
文献类型：
--
作者：
Alfson KJ;Avena LE;Beadles MW;Worwa G;Amen M;Patterson JL;Carrion R Jr;Griffiths A
丝状病毒埃博拉病毒（EBOV）和苏丹病毒（SUDV）可引起严重疾病，目前没有许可的对策可用于对抗它们。传播经常通过接触受感染者的体液发生。然而，很难确定某人何时或如何被感染，或者他们接触的传染性病毒的数量。有证据表明，感染剂量很低，但大多数已发表的研究使用高暴露剂量。本研究使用食蟹猴模型表征了暴露于低剂量EBOV或SUDV的结果。此外，由于当使用较低暴露剂量时，细胞培养物中病毒传代的影响可能更显著，因此使用具有野生型病毒特征（主要具有7-尿苷（7 U）基因型和高颗粒-空斑形成单位（PFU）比）或细胞培养传代病毒（主要具有8-尿苷（8 U）基因型，较低颗粒-PFU比）的病毒。与较高暴露剂量相比，低剂量暴露后的死亡时间延迟。这些数据表明，极低剂量的EBOV或SUDV足以引起致命疾病。低剂量暴露模型可以帮助为发病机制、传播和预防策略的优化研究提供信息。
The filoviruses Ebola virus (EBOV) and Sudan virus (SUDV) can cause severe diseases, and there are currently no licensed countermeasures available for use against them. Transmission occurs frequently via contact with bodily fluids from infected individuals. However, it can be difficult to determine when or how someone became infected, or the quantity of infectious virus to which they were exposed. Evidence suggests the infectious dose is low, but the majority of published studies use high exposure doses. This study characterized the outcome of exposure to a low dose of EBOV or SUDV, using a Macaca fascicularis model. Further, because the effect of virus passage in cell culture may be more pronounced when lower exposure doses are used, viruses that possessed either the characteristics of wild type viruses (possessing predominantly 7-uridine (7U) genotype and a high particle-to-plaque forming unit (PFU) ratio) or cell culture-passaged viruses (predominantly 8-uridine (8U) genotype, a lower particle-to-PFU ratio) were used. The time to death after a low dose exposure was delayed in comparison to higher exposure doses. These data demonstrated that an extremely low dose of EBOV or SUDV is sufficient to cause lethal disease. A low dose exposure model can help inform studies on pathogenesis, transmission, and optimization of prevention strategies.
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影响因子：
64.8
作者：
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DOI：
10.1073/pnas.93.8.3602
发表时间：
1996-04-16
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
Griffiths, Anthony