Expression patterns of bovine CD1 in vivo and assessment of the specificities of the anti-bovine CD1 antibodies.
Expression patterns of bovine CD1 in vivo and assessment of the specificities of the anti-bovine CD1 antibodies.
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DOI：
10.1371/journal.pone.0121923
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Van Rhijn I
中科院分区：
文献类型：
--
作者：
Nguyen TK;Reinink P;El Messlaki C;Im JS;Ercan A;Porcelli SA;Van Rhijn I
由于缺乏合适的动物模型，针对脂质、糖脂质和脂肽激活T细胞的体内效应的研究受到阻碍。小鼠和大鼠不表达CD1a、CD1b和CD1c分子，这些分子在人类中呈现病原体来源的脂质抗原。在牛中，两个CD1A和三个CD1B基因被转录。由这些基因编码的蛋白质在它们的抗原结合域和细胞质尾部上是不同的，这表明它们可能在细胞中以不同的方式运输，从而可以接触到不同的抗原。在目前的研究中，我们描述了牛CD1基因位点的基因组组织和牛CD1基因在新鲜分离的树突状细胞和来自不同组织的B细胞中的转录。通过检测重组单链牛CD1蛋白和转染CD1的细胞，我们确定了先前仅部分表征的抗CD1抗体的特异性，我们能够确定新分离细胞上细胞表面蛋白的表达。我们的研究表明，CD1b1和CD1b3比CD1b5表达更广泛，CD1a2比CD1a1表达更广泛。假传入淋巴树突状细胞表达CD1B基因，但在淋巴结中未检测到转录。尽管B细胞转录CD1B基因，但没有证据表明在细胞表面有蛋白表达。因此，CD1蛋白的表达模式在物种间很大程度上是保守的。
Research addressing the in vivo effects of T cell activation by lipids, glycolipids, and lipopeptides is hampered by the absence of a suitable animal model. Mice and rats do not express CD1a, CD1b, and CD1c molecules that present pathogen-derived lipid antigens in humans. In cattle, two CD1A and three CD1B genes are transcribed. The proteins encoded by these genes differ in their antigen binding domains and in their cytoplasmic tails, suggesting that they may traffic differently in the cell and thus have access to different antigens. In the current study, we describe the genomic organization of the bovine CD1 locus and transcription of bovine CD1 genes in freshly isolated dendritic cells and B cells from different tissues. After determining the specificity of previously only partly characterized anti-CD1 antibodies by testing recombinant single chain bovine CD1 proteins and CD1-transfected cells, we were able to determine cell surface protein expression on freshly isolated cells. Our study suggests that CD1b1 and CD1b3 are more broadly expressed than CD1b5, and CD1a2 is more broadly expressed than CD1a1. Pseudoafferent lymph dendritic cells express CD1B genes, but no transcription is detected in lymph nodes. Even though B cells transcribe CD1B genes, there is no evidence of protein expression at the cell surface. Thus, patterns of CD1 protein expression are largely conserved among species.
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影响因子：
3.2
作者：
van Beeck, Frank A. Looringh;Zajonc, Dirk M.;Moore, Peter F.;Schlotter, Yvette M.;Broere, Femke;Rutten, Victor P. M. G.;Willemse, Ton;Van Rhijn, Ildiko
通讯作者：
Van Rhijn, Ildiko
影响因子：
32.4
作者：
Winau, Florian;Hegasy, Guido;Kaufmann, Stefan H. E.
通讯作者：
Kaufmann, Stefan H. E.
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.4
作者：
Hayes, SM;Knight, KL
通讯作者：
Knight, KL
影响因子：
1.8
作者：
DUTIA, BM;HOPKINS, J
通讯作者：
HOPKINS, J