Two canine CD1a proteins are differentially expressed in skin.
Two canine CD1a proteins are differentially expressed in skin.
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DOI：
10.1007/s00251-008-0297-z
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发表时间：
2008-06
期刊：
影响因子：
3.2
通讯作者：
Van Rhijn, Ildiko
中科院分区：
文献类型：
--
作者：
van Beeck, Frank A. Looringh;Zajonc, Dirk M.;Moore, Peter F.;Schlotter, Yvette M.;Broere, Femke;Rutten, Victor P. M. G.;Willemse, Ton;Van Rhijn, Ildiko
脂质抗原通过CD1蛋白家族呈递给T细胞。在这项研究中，我们描述了完整的狗（Canis familiaris） CD1位点，它位于染色体38上。犬基因座包含8个CD1A基因（canCD1A），其中5个为假基因，1个为canCD1B， 1个为canCD1C， 1个为canCD1D， 1个为canCD1E。利用抗CD1单克隆抗体（mab），犬CD1蛋白在体内表达为canCD1a6、canCD1a8和canCD1b。CanCD1a6和canCD1a8被两种不同的单克隆抗体识别。此外，我们还发现了三个cand1a基因在犬类组织中的差异转录。在犬皮肤中，canCD1A8的转录水平高于canCD1A6，未检测到canCD1A2的转录。基于蛋白质建模和蛋白质序列比对，我们预测这两种犬CD1a蛋白可以在其凹槽中结合不同的糖脂。除了外畴结构的差异外，我们还观察到由canCD1A基因编码的三种细胞质尾部的独特存在。cDNA测序和表达序列标记序列证实存在一个短的、人类cd1a样的细胞质尾巴，长度为4个氨基酸，中间长度为15个氨基酸，长长度为31个氨基酸。
Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.
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