Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.
Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.
复制标题
DOI：
10.1371/journal.pone.0085364
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Grossi-de-Sa MF
中科院分区：
文献类型：
--
作者：
Antonino de Souza Júnior JD;Ramos Coelho R;Tristan Lourenço I;da Rocha Fragoso R;Barbosa Viana AA;Lima Pepino de Macedo L;Mattar da Silva MC;Gomes Carneiro RM;Engler G;de Almeida-Engler J;Grossi-de-Sa MF
根结线虫对全世界许多重要作物造成严重损害和产量损失。对南方根结线虫基因组的分析揭示了属于五种不同催化类别的大量蛋白酶。一些报告表明，南方根结线虫蛋白酶除了具有普通消化巨细胞内容物以供进食的功能外，还在线虫寄生中发挥重要作用。然而，这些蛋白质在寄生过程中的确切作用仍然未知，这使得它们成为基因沉默以解决蛋白质功能的有趣目标。在这项研究中，我们通过 RNAi 干扰敲低了天冬氨酸 (Mi-asp-1)、丝氨酸 (Mi-ser-1) 和半胱氨酸蛋白酶 (Mi-cpl-1)，以深入了解这些酶在宿主/线虫相互作用过程中的功能。产生了表达 Mi-ser-1 (dsSER)、Mi-cpl-1 (dsCPL) 和这三个基因一起 (dsFusion) 的 dsRNA 的烟草品系。虫瘿的组织学分析未显示巨细胞形态的明显差异。有趣的是，感染表达蛋白酶双链RNA的植物的线虫产卵数量减少。此外，在 dsSER 植物中成熟的线虫后代在卵孵化方面的成功率降低，而 dsCPL 和 dsFusion 植物产生的后代感染野生型宿主植物的成功率较低。定量 PCR 分析证实 Mi-cpl-1 和 Mi-ser-1 蛋白酶的转录物减少。我们的结果表明，这些蛋白酶可能参与线虫发育过程中的不同过程，如营养、繁殖和胚胎发生。更好地了解线虫蛋白酶及其在植物与线虫相互作用中可能发挥的作用可能有助于开发植物线虫控制的新工具。
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita proteases could play important roles in nematode parasitism, besides their function in ordinary digestion of giant cell contents for feeding. The precise roles of these proteins during parasitism however are still unknown, making them interesting targets for gene silencing to address protein function. In this study we have knocked-down an aspartic (Mi-asp-1), a serine (Mi-ser-1) and a cysteine protease (Mi-cpl-1) by RNAi interference to get an insight into the function of these enzymes during a host/nematode interaction. Tobacco lines expressing dsRNA for Mi-ser-1 (dsSER), Mi-cpl-1 (dsCPL) and for the three genes together (dsFusion) were generated. Histological analysis of galls did not show clear differences in giant cell morphology. Interestingly, nematodes that infected plants expressing dsRNA for proteases produced a reduced number of eggs. In addition, nematode progeny matured in dsSER plants had reduced success in egg hatching, while progeny resulting from dsCPL and dsFusion plants were less successful to infect wild-type host plants. Quantitative PCR analysis confirmed a reduction in transcripts for Mi-cpl-1 and Mi-ser-1 proteases. Our results indicate that these proteases are possibly involved in different processes throughout nematode development, like nutrition, reproduction and embryogenesis. A better understanding of nematode proteases and their possible role during a plant-nematode interaction might help to develop new tools for phytonematode control.
登录
查看更多内容
影响因子：
1.5
作者：
Britton, C;Murray, L
通讯作者：
Murray, L
影响因子：
4
作者：
Charlton, Wayne L.;Harel, H. Yael Meller;McPherson, Michael J.
通讯作者：
McPherson, Michael J.
DOI：
10.1074/jbc.m900519200
发表时间：
2009-06-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Dowen RH;Engel JL;Shao F;Ecker JR;Dixon JE
通讯作者：
Dixon JE
DOI：
10.1073/pnas.0604698103
发表时间：
2006-09-26
影响因子：
11.1
作者：
Huang, Guozhong;Allen, Rex;Hussey, Richard S.
通讯作者：
Hussey, Richard S.
影响因子：
2.2
作者：
BOLTER, CJ;JONGSMA, MA
通讯作者：
JONGSMA, MA