A beta-camera integrated with a microfluidic chip for radioassays based on real-time imaging of glycolysis in small cell populations.
A beta-camera integrated with a microfluidic chip for radioassays based on real-time imaging of glycolysis in small cell populations.
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基于小细胞群体糖酵解的实时成像，与微流体芯片集成的Beta相机。
DOI：
10.2967/jnumed.110.078725
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Chatziioannou AF
中科院分区：
文献类型：
--
作者：
Vu NT;Yu ZT;Comin-Anduix B;Søndergaard JN;Silverman RW;Chang CY;Ribas A;Tseng HR;Chatziioannou AF
开发了一种集成的β-相机和微流体芯片，其能够在小细胞群体中使用18F-FDG对糖酵解放射性测定进行定量成像，小细胞群体小到单个细胞。本文表明，集成系统使数字控制和定量测量的糖酵解在B-RafV 600 E突变的黑色素瘤细胞系响应特定的B-Raf抑制。β相机使用位置敏感的雪崩光电二极管来检测微流体芯片内的带电粒子发射探针。校准集成的β相机和微流控芯片系统，并使用4种不同的黑素瘤细胞系（M257、M202、M233和M229）测量线性度。微流控放射性测定用范围从数百个细胞到单个细胞的细胞群进行。M229细胞系具有纯合B-RafV 600 E突变，并且对B-Raf抑制剂PLX 4032高度敏感。在3天的过程中进行微流体放射性测定以评估PLX 4032对细胞18F-FDG摄取的细胞毒性。β照相机能够在微流控芯片中成像18F-FDG的放射性摄取。在放射性示踪剂孵育期间，使用37 MBq/mL的放射性浓度测量单细胞的18F-FDG摄取。对于体外细胞毒性监测，β相机显示，与溶剂对照相比，暴露于1 µM PLX 4032 3天降低了高敏感性M229细胞中每个细胞的18F-FDG摄取。集成的β相机和微流控芯片可以提供活细胞培养的数字控制，并允许同时对多个样品进行体外定量放射性测定。
An integrated β-camera and microfluidic chip was developed that is capable of quantitative imaging of glycolysis radioassays using 18F-FDG in small cell populations down to a single cell. This paper demonstrates that the integrated system enables digital control and quantitative measurements of glycolysis in B-RafV600E–mutated melanoma cell lines in response to specific B-Raf inhibition. The β-camera uses a position-sensitive avalanche photodiode to detect charged particle–emitting probes within a microfluidic chip. The integrated β-camera and microfluidic chip system was calibrated, and the linearity was measured using 4 different melanoma cell lines (M257, M202, M233, and M229). Microfluidic radioassays were performed with cell populations ranging from hundreds of cells down to a single cell. The M229 cell line has a homozygous B-RafV600E mutation and is highly sensitive to a B-Raf inhibitor, PLX4032. A microfluidic radioassay was performed over the course of 3 days to assess the cytotoxicity of PLX4032 on cellular 18F-FDG uptake. The β-camera is capable of imaging radioactive uptake of 18F-FDG in microfluidic chips. 18F-FDG uptake for a single cell was measured using a radioactivity concentration of 37 MBq/mL during the radiotracer incubation period. For in vitro cytotoxicity monitoring, the β-camera showed that exposure to 1 µM PLX4032 for 3 days decreased the 18F-FDG uptake per cell in highly sensitive M229 cells, compared with vehicle controls. The integrated β-camera and microfluidic chip can provide digital control of live cell cultures and allow in vitro quantitative radioassays for multiple samples simultaneously.
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影响因子：
6.1
作者：
Lavén, M;Velikyan, I;Wallenborg, S
通讯作者：
Wallenborg, S
DOI：
10.1016/s0168-9002(01)01724-7
发表时间：
2002-02-01
影响因子：
1.4
作者：
Bertolucci, E;Conti, M;Russo, P
通讯作者：
Russo, P
影响因子：
3.5
作者：
Russo, P.;Lauria, A.;Lastoria, S.
通讯作者：
Lastoria, S.
影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
3.5
作者：
Cabello, Jorge;Bailey, Alexis;Wells, Kevin
通讯作者：
Wells, Kevin