Non-invasive multiphoton imaging of extracellular matrix structures.
Non-invasive multiphoton imaging of extracellular matrix structures.
复制标题
DOI：
10.1002/jbio.200810045
复制
发表时间：
2008-12
影响因子：
2.8
通讯作者：
Schenke-Layland, Katja
中科院分区：
文献类型：
--
作者：
Schenke-Layland, Katja
关键词：
多光子显微镜已成为一种强大的方法，用于在其天然环境中对深层组织细胞和细胞外基质（ECM）结构进行无伪影、无损评价。通过与高度非中心对称的分子组装体（如纤维状胶原蛋白）相互作用，称为二次谐波产生（SHG）的非线性过程也已被证明是用于以亚微米分辨率原位可视化ECM隔室而不需要组织处理的重要诊断工具。这篇评论报告的应用多光子诱导的自发荧光和SHG显微镜，以确定胶原蛋白和弹性纤维的方向在本地，组织工程和加工，以及健康和患病的组织和器官。SHG信号分析用于量化各种心血管和外分泌组织以及软骨中的ECM损伤。这些新的成像方式打开了高分辨率原位和更重要的ECM结构，细胞和细胞内细胞器在活的完整组织中的体内成像的一般可能性。心脏瓣膜小叶具有独特的细胞外基质，其组织在不同的层中，可以通过多光子成像非破坏性地可视化。
Multiphoton microscopy has become a powerful method for the artifact-free, nondestructive evaluation of deep-tissue cells and extracellular matrix (ECM) structures in their native environment. By interacting with highly non-centrosymmetric molecular assemblies such as fibrillar collagen, the non-linear process called second harmonic generation (SHG) has also proven to be an important diagnostic tool for the visualization of ECM compartments in situ with submicron resolution without the need for tissue processing. This review reports on applications of multiphoton-induced autofluorescence and SHG microscopy to identify collagen and elastic fiber orientation in native, tissue-engineered and processed, as well as healthy and diseased, tissues and organs. SHG signal profiling was used to quantify ECM damage in various cardiovascular and exocrine tissues, as well as cartilage. These novel imaging modalities open the general possibility of high-resolution in situ and more important in vivo imaging of ECM structures, cells and intracellular organelles in living intact tissues. Heart valve leaflets have a unique extracellular matrix that is organized in distinct layers, which can be non-destructively visualized by multiphoton imaging.
登录
查看更多内容
影响因子：
37.8
作者：
Aikawa, E;Whittaker, P;Schoen, FJ
通讯作者：
Schoen, FJ
影响因子：
1.7
作者：
Del Gaudio, C.;Grigioni, M.;De Angelis, G.
通讯作者：
De Angelis, G.
影响因子：
14
作者：
König, K;Schenke-Layland, K;Stock, UA
通讯作者：
Stock, UA
影响因子：
2.8
作者：
Gray, Martha L.;Burstein, Deborah;Maroudas, Alice
通讯作者：
Maroudas, Alice
影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL