High-throughput sequencing of microRNAs in adenovirus type 3 infected human laryngeal epithelial cells.
High-throughput sequencing of microRNAs in adenovirus type 3 infected human laryngeal epithelial cells.
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3 型腺病毒感染的人喉上皮细胞中 MicroRNA 的高通量测序
DOI：
10.1155/2010/915980
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发表时间：
2010
影响因子：
--
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Qi Y;Tu J;Cui L;Guo X;Shi Z;Li S;Shi W;Shan Y;Ge Y;Shan J;Wang H;Lu Z
腺病毒感染可引起多种疾病，这取决于感染的血清型，如胃肠炎、结膜炎、膀胱炎和皮疹疾病，但感染机制仍不清楚。MicroRNA（miRNA）在细胞增殖、细胞分化和病毒感染等人类疾病的发病机制中发挥重要作用。我们使用SOLiD深度测序分析了来自腺病毒3型（AD3）感染的人喉上皮（Hep2）细胞的miRNA表达谱。在AD3感染的Hep2细胞中鉴定出492个前体miRNA，在对照中鉴定出540个前体miRNA。与对照组相比，在AD3感染的细胞中共有44种miRNAs表现出高表达，36种miRNAs表现出低表达。已经分析了miRNA的生物起源，并且通过定量PCR分析证实了一些SOLiD结果。本研究为进一步研究AD3感染的生物学功能提供了有益的线索。
Adenovirus infection can cause various illnesses depending on the infecting serotype, such as gastroenteritis, conjunctivitis, cystitis, and rash illness, but the infection mechanism is still unknown. MicroRNAs (miRNA) have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3) infected Human laryngeal epithelial (Hep2) cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.
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