Changes in cell morphology are coordinated with cell growth through the TORC1 pathway.
Changes in cell morphology are coordinated with cell growth through the TORC1 pathway.
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DOI：
10.1016/j.cub.2013.05.035
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发表时间：
2013-07-22
期刊：
影响因子：
9.2
通讯作者：
Amon, Angelika
中科院分区：
文献类型：
--
作者：
Goranov, Alexi I.;Gulati, Amneet;Dephoure, Noah;Takahara, Terunao;Maeda, Tatsuya;Gygi, Steven P.;Manalis, Scott;Amon, Angelika
生长速度不仅取决于细胞外信号（例如营养物质的可用性），还取决于细胞内过程。芽殖酵母细胞形态的变化（由肌动蛋白细胞骨架的极化介导）已被证明会减少细胞生长。在这里，我们证明肌动蛋白细胞骨架的极化抑制高度保守的雷帕霉素靶标复合物 1 (TORC1) 通路。这种下调通过 TORC1 途径调节 Iml1 复合物的失活而受到抑制，该复合物也在氮饥饿期间调节 TORC1。我们进一步证明，在长期极化生长条件后，生长减弱对于细胞恢复很重要。我们的结果表明，长时间的极化生长至少部分通过抑制 TORC1 途径来抑制蛋白质合成、质量积累和细胞大小的增加。我们推测这种机制可以协调细胞增大大小的能力与其生物合成能力。
Growth rate is determined not only by extracellular cues such as nutrient availability but also by intracellular processes. Changes in cell morphology in budding yeast, mediated by polarization of the actin cytoskeleton, have been shown to reduce cell growth. Here we demonstrate that polarization of the actin cytoskeleton inhibits the highly conserved Target of Rapamycin Complex 1 (TORC1) pathway. This downregulation is suppressed by inactivation of the TORC1 pathway regulatory Iml1 complex, which also regulates TORC1 during nitrogen starvation. We further demonstrate that attenuation of growth is important for cell recovery after conditions of prolonged polarized growth. Our results indicate that extended periods of polarized growth inhibit protein synthesis, mass accumulation, and the increase in cell size at least in part through inhibiting the TORC1 pathway. We speculate that this mechanism serves to coordinate the ability of cells to increase in size with their biosynthetic capacity.
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影响因子：
3.2
作者：
HARTWELL, LH
通讯作者：
HARTWELL, LH
影响因子：
16
作者：
Jacinto, E;Guo, B;Hall, MN
通讯作者：
Hall, MN
影响因子：
3.3
作者：
Loewith R;Hall MN
通讯作者：
Hall MN
DOI：
10.1073/pnas.77.4.1898
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LIAO, H;THORNER, J
通讯作者：
THORNER, J
影响因子：
64.8
作者：
Bishop, AC;Ubersax, JA;Shokat, KM
通讯作者：
Shokat, KM