Dietary Enterococcus faecalis LAB31 improves growth performance, reduces diarrhea, and increases fecal Lactobacillus number of weaned piglets.
Dietary Enterococcus faecalis LAB31 improves growth performance, reduces diarrhea, and increases fecal Lactobacillus number of weaned piglets.
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DOI：
10.1371/journal.pone.0116635
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liang Y
中科院分区：
文献类型：
--
作者：
Hu Y;Dun Y;Li S;Zhang D;Peng N;Zhao S;Liang Y
乳酸菌(LAB)被证明能提高断奶仔猪的生产性能。然而，很少有研究报道添加实验室粪肠球菌作为断奶仔猪促生长抗生素的替代品。本试验研究了粪肠球菌LAB31对断奶仔猪生产性能、腹泻发生率、血液指标、粪便细菌和乳杆菌群落的影响。将360头26±2日龄断奶仔猪随机分为5组，每组4头，每组20头。N组为阴性对照，不添加抗生素或益生菌；P组为硫酸新霉素100 mg/kg饲料；L、M、H组分别添加粪肠球菌LAB31 0.5×109、1.0×109、2.5×109CFU/kg饲料。H组的平均日增重和饲料转化效率显著高于N组，与P组差异显著(P<0.05)。此外，H组和P组的腹泻指数低于其他三组(P0<；0.05)。变性梯度凝胶电泳法(DGGE)显示，饲料中添加益生菌改变了细菌群落，其中M组的细菌多样性高于其他4组。实时定量聚合酶链式反应显示，添加益生菌后乳酸菌的相对数量增加，且H组高于N组(P0<；0.05)。然而，组特异性PCR-DGGE在乳酸菌组成和多样性方面五组间没有明显差异。因此，日粮中添加粪肠球菌LAB31可以改善断奶仔猪的生长性能，减少腹泻，增加粪便中乳酸菌的相对数量。
Lactic acid bacteria (LAB) have been shown to enhance performance of weaned piglets. However, few studies have reported the addition of LAB Enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. This study evaluated the effects of dietary E. faecalis LAB31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and Lactobacillus communities in weaned piglets. A total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to 5 groups (20 pens, with 4 pens for each group) for a trial of 28 days: group N (negative control, without antibiotics or probiotics); group P (Neomycin sulfate, 100 mg/kg feed); groups L, M and H (supplemented with E. faecalis LAB31 0.5×109, 1.0×109, and 2.5×109 CFU/kg feed, respectively). Average daily gain and feed conversion efficiency were found to be higher in group H than in group N, and showed significant differences between group H and group P (P0 < 0.05). Furthermore, groups H and P had a lower diarrhea index than the other three groups (P0 < 0.05). Denaturing gradient gel electrophoresis (DGGE) showed that the application of probiotics to the diet changed the bacterial community, with a higher bacterial diversity in group M than in the other four groups. Real-time PCR revealed that the relative number of Lactobacillus increased by addition of probiotics, and was higher in group H than in group N (P0 < 0.05). However, group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and diversity. Therefore, the dietary addition of E. faecalis LAB31 can improve growth performance, reduce diarrhea, and increase the relative number of Lactobacillus in feces of weaned piglets.
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影响因子：
4.1
作者：
Domann, Eugen;Hain, Torsten;Chakraborty, Trinad
通讯作者：
Chakraborty, Trinad
对大量人类肠道微生物群中抗生素抗性基因进行宏基因组分析
影响因子：
16.6
作者：
Hu, Yongfei;Yang, Xi;Zhu, Baoli
通讯作者：
Zhu, Baoli
DOI：
10.1111/j.1439-0450.1992.tb01169.x
发表时间：
1992-06-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
DECUPERE, F;DEPREZ, P;MUYLLE, E
通讯作者：
MUYLLE, E
影响因子：
3.3
作者：
Han, W.;Zhang, X. L.;Zhao, Y. X.
通讯作者：
Zhao, Y. X.
影响因子：
4.2
作者：
Janczyk, Pawel;Pieper, Robert;Souffrant, Wolfgang Bernhard
通讯作者：
Souffrant, Wolfgang Bernhard