Effect of proteolytic and detoxification enzyme inhibitors on Bacillus thuringiensis var. israelensis tolerance in the mosquito Aedes aegypti
Effect of proteolytic and detoxification enzyme inhibitors on Bacillus thuringiensis var. israelensis tolerance in the mosquito Aedes aegypti
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蛋白水解和解毒酶抑制剂对苏云金芽孢杆菌的影响
DOI：
10.1080/09583157.2016.1253828
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发表时间：
2017-02
影响因子：
1.4
通讯作者：
Lingling Zhang
中科院分区：
文献类型：
--
作者：
Xiong Guan;Shuangquan Zou;Lei Xu;Lingling Zhang
苏云金芽孢杆菌变种Israelensis（Bti）对蚊子幼虫具有高致病性，并被广泛用于蚊子控制。然而，与许多化学杀虫剂相比，其杀蚊活性相对较低。蚊子体内的解毒机制，除其他外，可能会中和Bti活性，导致抗性或耐受性。我们测试了针对化学杀虫剂的解毒机制是否也可能对Bti起作用，使其效果降低。我们针对埃及伊蚊幼虫中参与解毒的四种酶与已用于化学杀虫剂抗性研究的抑制剂，并测定其对Bti毒性的影响。结果表明，苯甲磺酰氟（PMSF），马来酸二乙酯，苯巴比妥（PB），胡椒基丁醚（PBO）改变Bti毒性不同程度。PMSF是一种丝氨酸蛋白酶抑制剂，可防止Bti消化并提高Bti活性。诱导几种解毒酶的PB根据治疗方法有两种不同的效果。PB治疗不连续时死亡率较高（149%），而连续治疗则较低（101%）。PBO是一种典型的细胞色素P450抑制剂，可增加Bti效应（159%）。不连续预处理的幼虫与PB，然后PBO的组合具有协同效应，并显示出增加的活性（146%）。看来蚊子对Bti的抗性机制与化学杀虫剂的抗性机制相似。我们的研究表明，我们可能能够通过抑制某些解毒系统来增加Bti活性，这些解毒系统与广谱化学杀虫剂一样活跃。
ABSTRACT Bacillus thuringiensis var. israelensis (Bti) is highly pathogenic to mosquito larvae and is widely used for mosquito control. Its mosquitocidal activity however is relatively low compared to many chemical insecticides. The detoxification mechanisms in the mosquito, among other things, might neutralize the Bti activity, resulting in resistance or tolerance. We tested whether or not the detoxification mechanisms against chemical insecticides might also operate against Bti, rendering it less effective. We targeted four enzymes in Aedes aegypti larvae involved in detoxification with inhibitors that have been used in resistance studies in chemical insecticides and assayed their effects on Bti toxicity. Results revealed that phenylmethanesulphonyl fluoride (PMSF), diethyl maleate, phenobarbital (PB), and piperonyl butoxide (PBO) altered Bti toxicity to various degrees. PMSF is a serine protease inhibitor that prevents Bti digestion and improves Bti activity. PB that induces several detoxifying enzymes had two different effects depending on the method of treatment. Mortality was higher when treatment with PB was discontinuous (149%) whereas with continuous treatment it was lower (101%). PBO, a typical cytochrome P450 inhibitor, increased Bti effect (159%). The combination of discontinuous pretreatment of larvae with PB followed by PBO had a synergistic effect and showed increased activity (146%). It appears that the mechanism for Bti resistance in mosquitoes is similar to that of chemical insecticides. Our studies indicate that we may be able to increase Bti activity by inhibiting some of the detoxification systems as active as broad spectrum chemical insecticides.
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影响因子：
3.8
作者：
Li, HR;Oppert, B;Buschman, LL
通讯作者：
Buschman, LL
影响因子：
4.1
作者：
Vontas, JG;Small, GJ;Hemingway, J
通讯作者：
Hemingway, J
影响因子：
3.3
作者：
Despres, Laurence;Stalinski, Renaud;David, Jean-Philippe
通讯作者：
David, Jean-Philippe
影响因子：
4.1
作者：
S. Sumner;T. Fennell;T. Moore;B. Chanas;F. J. Gonzalez;B. Ghanayem
通讯作者：
S. Sumner;T. Fennell;T. Moore;B. Chanas;F. J. Gonzalez;B. Ghanayem
影响因子：
4.5
作者：
G. Tetreau;A. Chandor-Proust;Frédéric Faucon;Renaud Stalinski;Idir Akhouayri;S. Prud'homme;M. Raveton;S. Reynaud
通讯作者：
G. Tetreau;A. Chandor-Proust;Frédéric Faucon;Renaud Stalinski;Idir Akhouayri;S. Prud'homme;M. Raveton;S. Reynaud