Chytridiomycosis Outbreak in a Chilean Giant Frog (Calyptocephalella gayi) Captive Breeding Program: Genomic Characterization and Pathological Findings.
Chytridiomycosis Outbreak in a Chilean Giant Frog (Calyptocephalella gayi) Captive Breeding Program: Genomic Characterization and Pathological Findings.
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DOI：
10.3389/fvets.2021.733357
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发表时间：
2021
影响因子：
3.2
通讯作者：
Azat C
中科院分区：
文献类型：
--
作者：
Alvarado-Rybak M;Acuña P;Peñafiel-Ricaurte A;Sewell TR;O'Hanlon SJ;Fisher MC;Valenzuela-Sánchez A;Cunningham AA;Azat C
野生动物中新出现的传染病越来越多地与动物死亡和物种减少有关，但其来源和遗传特征往往仍然难以捉摸。两栖类壶菌病，由真菌Batrachochytrium dendrobatim（Bd）引起，已与灾难性的和有充分记录的两栖类种群下降和灭绝在全球范围内。我们使用组织学和全基因组测序来描述的病变所造成的，和遗传变异，两个Bd分离物获得的大规模死亡事件中的圈养人口的威胁智利巨蛙（Calyptocephalella gayi）。这是第一次在这种魅力和下降的青蛙物种中检测到Bd和高死亡率之间的关联。病理学检查显示，30只死亡的变态青蛙出现无颌症或短颌症，这是首次报道的与壶菌病相关的疾病。系统基因组分析表明，从圈养的C. gayi组与其他Bd分离株（AVS 2，AVS 4和AVS 7）形成一个单一的高度支持的智利Bd分支内的全球泛动物谱系的Bd（BdGPL）。这些发现对于加强生物安全措施以防止壶菌病对其他地方圈养繁殖计划的影响具有重要意义。
Emerging infectious diseases in wildlife are increasingly associated with animal mortality and species declines, but their source and genetic characterization often remains elusive. Amphibian chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), has been associated with catastrophic and well-documented amphibian population declines and extinctions at the global scale. We used histology and whole-genome sequencing to describe the lesions caused by, and the genetic variability of, two Bd isolates obtained from a mass mortality event in a captive population of the threatened Chilean giant frog (Calyptocephalella gayi). This was the first time an association between Bd and high mortality had been detected in this charismatic and declining frog species. Pathological examinations revealed that 30 dead metamorphosed frogs presented agnathia or brachygnathia, a condition that is reported for the first time in association with chytridiomycosis. Phylogenomic analyses revealed that Bd isolates (PA1 and PA2) from captive C. gayi group with other Bd isolates (AVS2, AVS4, and AVS7) forming a single highly supported Chilean Bd clade within the global panzootic lineage of Bd (BdGPL). These findings are important to inform the strengthening of biosecurity measures to prevent the impacts of chytridiomycosis in captive breeding programs elsewhere.
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影响因子：
2.6
作者：
James TY;Toledo LF;Rödder D;da Silva Leite D;Belasen AM;Betancourt-Román CM;Jenkinson TS;Soto-Azat C;Lambertini C;Longo AV;Ruggeri J;Collins JP;Burrowes PA;Lips KR;Zamudio KR;Longcore JE
通讯作者：
Longcore JE
影响因子：
1.3
作者：
Andre, Sara E.;Parker, John;Briggs, Cheryl J.
通讯作者：
Briggs, Cheryl J.
影响因子：
6
作者：
Fisher, Matthew C.;Garner, Tyenton W. J.
通讯作者：
Garner, Tyenton W. J.
影响因子：
16.6
作者：
Bates KA;Clare FC;O'Hanlon S;Bosch J;Brookes L;Hopkins K;McLaughlin EJ;Daniel O;Garner TWJ;Fisher MC;Harrison XA
通讯作者：
Harrison XA
影响因子：
4.9
作者：
Bataille, Arnaud;Fong, Jonathan J.;Waldman, Bruce
通讯作者：
Waldman, Bruce