PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy.
PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy.
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PTX3：甲状腺相关眼病的潜在生物标志物
DOI：
10.1155/2018/5961974
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发表时间：
2018
影响因子：
--
通讯作者：
Wei R
中科院分区：
文献类型：
--
作者：
Mou P;Chen Z;Jiang L;Cheng J;Wei R
背景甲状腺相关眼病(TAO)是一种自身免疫性疾病，涉及炎症和组织重塑。五角蛋白-3(PTX3)是天然免疫系统的一种成分，最近发现与自身免疫有关。提示PTX3可能参与了TAO的炎症过程。方法所有研究对象均为TAO患者和健康对照组(共45：28)。采用RNA-SEQ技术检测眼眶脂肪结缔组织的差异基因表达。用定量聚合酶链式反应对结果进行验证。免疫组织化学(IHC)法检测眼眶脂肪结缔组织中PTX3蛋白的表达。采用酶联免疫吸附试验(ELISA法)测定血清中PTX3浓度。结果1.86logPTX2Fc在组眼眶脂肪结缔组织中的表达明显高于对照组(FDR=0.0059)。定量聚合酶链式反应证实了这种差异(增加了5.59倍，p=0.0012)。证明了PTX3蛋白的存在。正常对照组眼眶脂肪组织呈PTX3弱阳性表达，TAO组呈强阳性表达。与对照组相比，患者血清甲状旁腺素3浓度显著升高(增加1.9%；p<；0.0001)。结论TAO患者眼眶组织和血清中PTX3表达增加，提示PTX3与TAO之间存在潜在的关系。
Background Thyroid associated ophthalmopathy (TAO) is an autoimmune disease, which involves inflammation and tissue remodeling. Pentraxin-3 (PTX3) is a component of innate immune system and recently implicated in autoimmunity. This observation may indicate that PTX3 participates in the inflammatory process of TAO. Methods All studies were performed on TAO patients and healthy controls (45: 28 in total). RNA-seq was used to detect differential gene expression of orbital adipose-connective tissue. Quantitative PCR was performed to verify the results. PTX3 protein in orbital adipose-connective tissues was visualized by immunohistochemistry (IHC). PTX3 concentration in serum was determined by enzyme-linked immunosorbent assay (ELISA). Results RNA-seq showed 1.86-log2FC higher PTX3 expression in the orbital adipose-connective tissues from TAO group than controls (FDR = 0.0059). qPCR confirmed the difference (5.59-fold increase, p = 0.0012). The presence of PTX3 protein was demonstrated. Orbital adipose tissue from healthy controls showed weak staining for PTX3 while tissue from TAO group was strongly positive. Serum PTX3 concentration was significantly elevated in patients when compared to the control group (1.9-fold increase; p < 0.0001). Conclusions Patients with TAO showed increased presence of PTX3 in orbital tissue and serum, which may suggest a potential relationship of PTX3 and TAO.
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