Early biofilm and streamer formation is mediated by wall shear stress and surface wettability: A multifactorial microfluidic study.
Early biofilm and streamer formation is mediated by wall shear stress and surface wettability: A multifactorial microfluidic study.
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DOI：
10.1002/mbo3.1310
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发表时间：
2022-08
期刊：
影响因子：
3.4
通讯作者：
Salta, Maria
中科院分区：
文献类型：
--
作者：
Chun, Alexander L. M.;Mosayyebi, Ali;Butt, Arthur;Carugo, Dario;Salta, Maria
关键词：
生物膜是复杂的微生物群落，封装在自产的聚合物外基质 (EPS) 内，形成复杂的三维结构，允许液体和营养物质通过它们运输。这些聚集为组成微生物提供了增强的保护，使其免受环境刺激（如流体流动）的影响，并且还与抗菌化合物的更高耐药性有关，引起了海洋（生物污垢和水产养殖）、医疗（感染和抗菌素耐药性）、牙科（牙菌斑）、食品安全等众多领域的持续关注，并导致能量损失和腐蚀。最近的研究表明，生物膜与微塑料相互作用，通常会影响它们达到更高营养水平的途径。先前的研究表明，最初的细菌附着受到表面特性的影响。使用微流体流动池，我们研究了两种物种（Cobetia marina 和 Pseudomonas aeruginosa）生物膜形成时壁剪切应力 (τw) 和表面特性（表面润湿性）之间的关系。我们使用核酸染色和终点共焦激光扫描显微镜研究了低密度聚乙烯 (LDPE) 膜、Permanox® 载玻片和载玻片上生物膜的形成。结果表明，流动条件影响生物膜的生物量、最大厚度和表面积，较高的 τw (5.6Pa) 会比较低的 τw (0.2Pa) 产生更薄的生物膜。此外，我们观察到测试表面的生物膜形成存在差异，与 Permanox® 和玻璃相比，LDPE 通常表现出更多的整体生物膜。此外，我们证明了在层流条件下直微通道内生物膜流的形成。我们使用微流体平台通过同时施加四种不同的壁剪切应力来研究生物膜的发育，并测试了具有不同疏水性的三种不同表面。我们的主要结果表明，表面润湿性在低剪切应力下的 Cobetia marina 和铜绿假单胞菌生物膜形成中发挥着作用。然而，随着剪切应力的增加，这种情况被最小化。在最高剪切应力下观察到生物膜流光。
Biofilms are intricate communities of microorganisms encapsulated within a self‐produced matrix of extra‐polymeric substances (EPS), creating complex three‐dimensional structures allowing for liquid and nutrient transport through them. These aggregations offer constituent microorganisms enhanced protection from environmental stimuli—like fluid flow—and are also associated with higher resistance to antimicrobial compounds, providing a persistent cause of concern in numerous sectors like the marine (biofouling and aquaculture), medical (infections and antimicrobial resistance), dentistry (plaque on teeth), food safety, as well as causing energy loss and corrosion. Recent studies have demonstrated that biofilms interact with microplastics, often influencing their pathway to higher trophic levels. Previous research has shown that initial bacterial attachment is affected by surface properties. Using a microfluidic flow cell, we have investigated the relationship between both wall shear stress (τw) and surface properties (surface wettability) upon biofilm formation of two species (Cobetia marina and Pseudomonas aeruginosa). We investigated biofilm development on low‐density polyethylene (LDPE) membranes, Permanox® slides, and glass slides, using nucleic acid staining and end‐point confocal laser scanning microscopy. The results show that flow conditions affect biomass, maximum thickness, and surface area of biofilms, with higher τw (5.6 Pa) resulting in thinner biofilms than lower τw (0.2 Pa). In addition, we observed differences in biofilm development across the surfaces tested, with LDPE typically demonstrating more overall biofilm in comparison to Permanox® and glass. Moreover, we demonstrate the formation of biofilm streamers under laminar flow conditions within straight micro‐channels. We used a microfluidic platform to investigate biofilm development by applying four different wall shear stresses simultaneously and tested three different surfaces with varying hydrophobicity. Our key results showed that surface wettability played a role in biofilm formation for Cobetia marina and Pseudomonas aeruginosa at low shear stress. However, this was minimized as the shear stress increased. Biofilm streamers were observed at the highest shear stress.
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影响因子：
6
作者：
Deng, JP;Yang, WT;Rånby, B
通讯作者：
Rånby, B
DOI：
10.1073/pnas.1300321110
发表时间：
2013-03-12
影响因子：
11.1
作者：
Drescher, Knut;Shen, Yi;Stone, Howard A.
通讯作者：
Stone, Howard A.
DOI：
10.1016/j.ijadhadh.2014.01.027
发表时间：
2014-04-01
影响因子：
3.4
作者：
Bohinc, Klemen;Drazic, Goran;Raspor, Peter
通讯作者：
Raspor, Peter
影响因子：
2.9
作者：
Jeong, Heon-Ho;Jeong, Seong-Geun;Lee, Chang-Soo
通讯作者：
Lee, Chang-Soo
影响因子：
4.4
作者：
Kim, Suran;Li, Xi-Hui;Lee, Joon-Hee
通讯作者：
Lee, Joon-Hee