A high seroprevalence of antibodies to pertussis toxin among Japanese adults: Qualitative and quantitative analyses.
A high seroprevalence of antibodies to pertussis toxin among Japanese adults: Qualitative and quantitative analyses.
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DOI：
10.1371/journal.pone.0181181
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kamachi K
中科院分区：
文献类型：
--
作者：
Moriuchi T;Otsuka N;Hiramatsu Y;Shibayama K;Kamachi K
2013年，全国血清监测发现，日本成年人百日咳博德泰拉百日咳毒素（PT）抗体的血清阳性率很高。因此，我们旨在确定这种高血清阳性率的原因，并分析成人（35-44岁）、幼儿（4-7岁）和较大儿童（10-14岁）中PT和丝状血凝素（FHA）抗体的滴度。我们的定量分析显示，与年幼和年长的儿童相比，成人血清中抗pt IgG和pt中和抗体的流行率更高，抗fha IgG的滴度相似。各年龄组pt中和抗体滴度与抗pt IgG滴度呈正相关（rs值为0.326 ~ 0.522），随年龄增长相关性有降低的趋势。当我们比较成人血清和纯化IgG时，pt中和抗体与抗pt IgG的比例有显著差异（p = 0.016），尽管在幼儿和大龄儿童中没有观察到这一结果。因此，一些成年人似乎对PT具有非IgG免疫球蛋白。我们的分析还显示，成人具有高贪婪度的抗PT IgG（贪婪指数：63.5%，在儿童中观察到类似的结果）；成人抗fha IgG阳性率为37.9% （p < 0.05）。低亲和力抗fha IgG可能与其他呼吸道病原体（例如，副百日咳博德氏菌、流感嗜血杆菌或肺炎支原体）的感染有关，这些病原体会产生fha样蛋白的抗体。我们的观察表明，这些成年人在成年期间感染过百日咳和其他病原体。
In 2013, national serosurveillance detected a high seroprevalence of antibodies to pertussis toxin (PT) from Bordetella pertussis among Japanese adults. Thus, we aimed to determine the cause(s) of this high seroprevalence, and analyzed the titers of antibodies to PT and filamentous hemagglutinin (FHA) among adults (35–44 years old), young children (4–7 years old), and older children (10–14 years old). Our quantitative analyses revealed that adults had higher seroprevalences of anti-PT IgG and PT-neutralizing antibodies, and similar titers of anti-FHA IgG, compared to the young and older children. Positive correlations were observed between the titers of PT-neutralizing antibodies and anti-PT IgG in all age groups (rs values of 0.326–0.522), although the correlation tended to decrease with age. The ratio of PT-neutralizing antibodies to anti-PT IgG was significantly different when we compared the serum and purified IgG fractions among adults (p = 0.016), although this result was not observed among young and older children. Thus, it appears that some adults had non-IgG immunoglobulins to PT. Our analyses also revealed that adults had high-avidity anti-PT IgG (avidity index: 63.5%, similar results were observed among the children); however, the adults had lower-avidity anti-FHA IgG (37.9%, p < 0.05). It is possible that low-avidity anti-FHA IgG is related to infection with other respiratory pathogens (e.g., Bordetella parapertussis, Haemophilus influenzae, or Mycoplasma pneumoniae), which produces antibodies to FHA-like proteins. Our observations suggest that these adults had been infected with B. pertussis and other pathogen(s) during their adulthood.
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DOI：
10.1111/j.1600-0463.2010.02664.x
发表时间：
2010-12
期刊：
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
影响因子：
--
作者：
Dalby T;Sørensen C;Petersen JW;Krogfelt KA
通讯作者：
Krogfelt KA
DOI：
10.1007/s10096-010-1104-y
发表时间：
2011-03
影响因子：
4.5
作者：
Guiso, N.;Berbers, G.;Fry, N. K.;He, Q.;Riffelmann, M.;von Koenig, C. H. Wirsing
通讯作者：
von Koenig, C. H. Wirsing
影响因子：
3.1
作者：
BARENKAMP, SJ;LEININGER, E
通讯作者：
LEININGER, E
影响因子：
5.5
作者：
Jogi, Piia;Oona, Marje;Lutsar, Irja
通讯作者：
Lutsar, Irja
影响因子：
5.5
作者：
Konda, T;Kamachi, K;Matsunaga, Y
通讯作者：
Matsunaga, Y