Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay.
Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay.
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DOI：
10.1111/j.1600-0463.2010.02664.x
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Krogfelt KA
中科院分区：
文献类型：
--
作者：
Dalby T;Sørensen C;Petersen JW;Krogfelt KA
首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容百日咳血清学：评价免疫球蛋白抗PT酶联免疫吸附试验替代CHO细胞试验。APMIS2010；118：968-72。评价百日咳疫苗诱导抗体的血清学试验有两种：中国仓鼠卵巢细胞(CHO细胞)试验和免疫球蛋白G(Ig G)抗百日咳毒素(PT)酶联免疫吸附试验(Ig G anti-PT EL ISA)。最近，这两种技术都进行了改进，以提高与具有干扰活性的血清(CHO细胞试验)或加热处理的血清(Ig G抗PT-EL ISA)的性能。通过分析来自先前临床试验的100个个体血清样本和来自最近接种丹麦无细胞百日咳疫苗的20名丹麦成年人的纵向血清收集的213个血清样本，对这两种改进的技术进行了比较。比较表明，两种分析方法的结果之间存在显著的线性相关性，对100个个体样本的p值为0.0001。因此，我们得出结论，改进的免疫球蛋白抗PT-ELISA可以替代通常麻烦和耗时的CHO细胞法来检测疫苗诱导的人抗PT抗体。
Dalby T, Sørensen C, Petersen JW, Krogfelt KA. Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay. APMIS 2010; 118: 968–72. Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of <0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.
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影响因子：
3.6
作者：
Carlsson, RM;Claesson, BA;Lundin, C
通讯作者：
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影响因子：
5.5
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DOI：
10.1007/bf02097184
发表时间：
1987-02-01
影响因子：
4.5
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
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