Genomic characterization of enterohaemolysin-encoding haemolytic Escherichia coli of animal and human origin.
Genomic characterization of enterohaemolysin-encoding haemolytic Escherichia coli of animal and human origin.
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DOI：
10.1099/mgen.0.000999
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发表时间：
2023-04
影响因子：
3.9
通讯作者：
Kolenda, Rafat
中科院分区：
文献类型：
--
作者：
Sidorczuk, Katarzyna;Aleksandrowicz, Adrianna;Burdukiewicz, Michat;Kingsley, Robert A.;Kolenda, Rafat
肠溶血素 (Ehx) 和 α-溶血素是引起大肠杆菌溶血表型的毒力相关因子 (VAF)。研究表明，染色体和质粒编码的α-溶血素是特定致病型、毒力相关因子和宿主的特征。然而，α-溶血素和肠溶血素的流行率在大多数致病型中并不重叠。因此，本研究的重点是与人类和动物传染病多种致病型相关的溶血性大肠杆菌群体的特征。使用基因组学方法，我们研究了肠溶血素编码菌株的特征，以确定区分肠溶血素阳性和α溶血素阳性大肠杆菌群体的因素。为了阐明 Ehx 亚型的功能，我们分析了 Ehx 编码基因并推断了 EhxA 系统发育。这两种溶血素与不同的粘附素、铁吸收或毒素系统有关。 α-溶血素主要存在于尿路致病性大肠杆菌 (UPEC) 中，并预测为染色体编码，或非致病性和未确定的大肠杆菌致病型，通常预测为质粒编码。肠溶血素主要与产志贺毒素大肠杆菌 (STEC) 和肠出血性大肠杆菌 (EHEC) 相关，预计由质粒编码。这两种类型的溶血素均存在于非典型肠病性大肠杆菌 (aEPEC) 中。此外，我们还发现了一种新的 EhxA 亚型，仅存在于基因组中，具有非致病性大肠杆菌的 VAF 特征。这项研究揭示了不同致病型溶血大肠杆菌之间的复杂关系，为理解溶血素在发病机制中的潜在作用提供了一个框架。
Enterohaemolysin (Ehx) and alpha-haemolysin are virulence-associated factors (VAFs) causing the haemolytic phenotype in Escherichia coli . It has been shown that chromosomally and plasmid-encoded alpha-haemolysin are characteristic of specific pathotypes, virulence-associated factors and hosts. However, the prevalence of alpha- and enterohaemolysin does not overlap in the majority of pathotypes. Therefore, this study focuses on the characterization of the haemolytic E. coli population associated with multiple pathotypes in human and animal infectious diseases. Using a genomics approach, we investigated characteristic features of the enterohaemolysin-encoding strains to identify factors differentiating enterohaemolysin-positive from alpha-haemolysin-positive E. coli populations. To shed light on the functionality of Ehx subtypes, we analysed Ehx-coding genes and inferred EhxA phylogeny. The two haemolysins are associated with a different repertoire of adhesins, iron acquisition or toxin systems. Alpha-haemolysin is predominantly found in uropathogenic E. coli (UPEC) and predicted to be chromosomally encoded, or nonpathogenic and undetermined E. coli pathotypes and typically predicted to be plasmid-encoded. Enterohaemolysin is mainly associated with Shiga toxin-producing E. coli (STEC) and enterohaemorrhagic E. coli (EHEC) and predicted to be plasmid-encoded. Both types of haemolysin are found in atypical enteropathogenic E. coli (aEPEC). Moreover, we identified a new EhxA subtype present exclusively in genomes with VAFs characteristic of nonpathogenic E. coli . This study reveals a complex relationship between haemolytic E. coli of diverse pathotypes, providing a framework for understanding the potential role of haemolysin in pathogenesis.
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中国非O157产志贺毒素大肠杆菌菌株肠溶血素基因（ehxA）的遗传多样性
影响因子：
4.6
作者：
Fu S;Bai X;Fan R;Sun H;Xu Y;Xiong Y
通讯作者：
Xiong Y
影响因子：
4.4
作者：
Lorenz, Sandra C.;Son, Insook;Kase, Julie A.
通讯作者：
Kase, Julie A.
影响因子：
14.9
作者：
Galata V;Fehlmann T;Backes C;Keller A
通讯作者：
Keller A
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
3.3
作者：
Delicato, ER;de Brito, BG;Vidotto, MC
通讯作者：
Vidotto, MC